Chromatographia

, Volume 57, Supplement 1, pp S103–S113 | Cite as

Derivatization, stability and chromatographic behavior ofo-phthaldialdehyde amino acid and amine derivatives:o-Phthaldialdehyde/ 2-mercaptoethanol reagent

  • R. Hanczkó
  • I. Molnár-Perl
Originals Column Liquid Chromatography

Summary

A literature overview is given of HPLC methods currently in use to determine amino acids (AAs) and amines (As) as theiro-phthaldialdyde (OPA)/2-mercaptoethanol (MCE) derivatives. This is followed by an exhaustive derivatization study performed using the classical proteinogen AAs, C1–C4 aliphatic As, several polyamines (PAs) and the OPA/MCE reagent. Model studies have been carried out as a function of reaction time and reagent composition. The primary importance of reagent composition (mole ratios of OPA to SH-group additive) is repeatedly proven. Stabilities of the OPA/MCE derivatives have been compared with corresponding OPA/3-mercaptopropionic acid (MPA) and/or OPA/N-acetyl-L-cysteine (NAC) compounds, obtained under the same conditions.

Crucial factors proven to influence the stability of OPA derivatives of AAs and As are discussed in detail: (i) preparation and storage conditions of the OPA reagents, (ii) special behavior of the currently believed to be particularly unstable, AAs and As associated with their original molecular structure, e.g. glycine, β-alanine, γ-amino butyric acid (GABA), histidine, ornithine and lysine, several As, as well as (iii) the mole ratios of the OPA/SH-additive/AA and/or A.

Key Words

Column liquid chromatography o-Phthaldialdehyde-amino acid and amine derivatives Stability studies and comparisons 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • R. Hanczkó
    • 1
  • I. Molnár-Perl
    • 1
  1. 1.Institute of Inorganic & Analytical ChemistryL. Eötvös UniversityBudapest 112

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