, Volume 56, Issue 5–6, pp 345–349 | Cite as

Fractionation of Bulgarian viper (Vipera ammodytes) venoms. Relation of venom content and subspecies affiliation of the snakes

  • V. Bardarov
  • B. Aleksiev
Originals Column Liquid Chromatography


A cation-exchange procedure has been developed for fractionation of venom from Bulgarian snakes (Vipera ammodytes), using a 75×7.5 mm TSK SP-5 PW column for gradient elution of venom components with NH4OAc and UV-detection at 280 nm. The procedure developed was applied for venom component separation and especially to assay the vipoxin content—a previously studied and described toxic protein complex—in the venom of various subspecies of Bulgarianvipera ammodytes. Chromatographic profiles obtained show that venom fromVipera ammodytes ssp.montandoni Boulenger, 1904 andVipera ammodytes ssp.meridionalis Boulenger, 1903 contain vipoxin (content higher in venom ofVipera ammodytes ssp.meridionalis), while in the venom ofVipera ammodytes ssp.ammodytes Linnaeus, 1758 vipoxin is absent.

All data obtained show that the procedure developed can be successfully used for reliable subspecies affiliation by venom analysis and assay of vipoxin (in analytical mode) and its purification (in semipreparative mode) also.

Key Words

Column liquid chromatography Cation exchange Snake venom Vipoxin Vipera ammodytes subspecies 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • V. Bardarov
    • 1
  • B. Aleksiev
    • 2
  1. 1.Toxicochemical lab. Department of ToxicologyMilitary Medical AcademySofiaBulgaria
  2. 2.Technological CollegeRazgradBulgaria

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