Summary
The temperature-dependent separation of bryostatins by HPLC was examined on an octadecyl bonded stationary phase, using column temperatures between 0 and 40°C and mobile phase temperatures from 0 to 25°C. The retention time and resolution of bryostatins changed drastically and separation improved with decreasing temperature. A column temperature of less than 5°C and a mobile phase temperature of less than 15°C is recommended for a good resolution of bryostatins for routine work.
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Lu, F., Yang, G.J., Liu, L.L. et al. Temperature-dependent separation of bryostatin 18 and 10 by high performance liquid chromatography. Chromatographia 54, 57–59 (2001). https://doi.org/10.1007/BF02491833
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DOI: https://doi.org/10.1007/BF02491833