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Determination of baclofen by derivatization with anthracene-2,3-dicarboxaldehyde followed by capillary electrophoresis with laser-induced fluorescence detection

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Summary

A new derivatization method has been developed for the sensitive and selective determination of baclofen (4-amino-3-p-chlorophenylbutyric acid). Anthracene-2,3-dicarboxaldehyde (ADA) was employed to form a stable conjugate of baclofen whose excitation wavelength is red-shifted from the original reagent. The ADA-baclofen derivative was separated by capillary electrophoresis and detected with a green HeNe laser-induced fluorescence detector. Linearity of more than two orders of magnitudes was generally obtained. The concentration limit of detection (CLOD) was in the 10-8M range. A derivatization limit of detection of 0.3μM was achieved for baclofen. The precisions of the migration times and peak areas were 1.0% and 4.2%, respectively. The applicability of the method for the determination of baclofen in human plasma was examined.

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References

  1. Knutsson, E.; Lindblom, U.; Martensson, A.J. Neurol. Sci. 1974,23, 473–484.

    Article  CAS  Google Scholar 

  2. Degen, P.H.; Riess, W.J. Chromatogr. 1976,117, 399–405.

    Article  CAS  Google Scholar 

  3. Kochak, G.; Hone, F.J. Chromatogr. 1984,310, 319–326.

    CAS  Google Scholar 

  4. Swahn, C.; Beving, H.; Sedvall, G.J. Chromatogr. 1979,162, 433–438.

    CAS  Google Scholar 

  5. Harrison, P.M.; Tonkin, A.M.; McLean, A.J.J. Chromatogr. 1985,339, 424–428.

    CAS  Google Scholar 

  6. Wuis, E.W.; Van Beijsterveldt, L.E.C.; Dirks, R.J.M.; Vree, T.B.; Van Der Kleyn E.J. Chromatogr. 1987,420, 212–216.

    CAS  Google Scholar 

  7. Rustum, A.M.J. Chromatogr. 1989,487, 107–115.

    CAS  Google Scholar 

  8. Shimada, K.; Mitamura, K.; Morita, M.; Hirakata, K.J. Liq. Chromatogr. A 1993,16, 3311–3320.

    CAS  Google Scholar 

  9. Millerioux, L.; Brault, M.; Gualano, V.; Mignot, A.J. Chromatogr. A 1996,729, 309–314.

    Article  CAS  Google Scholar 

  10. Wuis, E.W.; Dirks, R.J.M.; Vree, T.B.; Van Der Kleyn, E.J. Chromatogr. 1985,337, 341–350.

    CAS  Google Scholar 

  11. Wuis, E.W.; Beneken Kolmer, E.W.J.; Van Beijsterveldt, L.E.C.; Burgers, R.C.M.; Vree, T.B.; Van Der Kleyn, E.J. Chromatogr. 1987,415, 419–422.

    CAS  Google Scholar 

  12. Tosunoglu, S.; Ersoy, L.Analyst 1995,120, 373–375.

    Article  CAS  Google Scholar 

  13. Flardh, M.; Jacobson, B.J. Chromatogr. A 1999,846, 169–173.

    Article  CAS  Google Scholar 

  14. Chiang, M.; Chang, S.Y.; Whang, C.J. Chromatogr. A 2000,877, 233–237.

    Article  CAS  Google Scholar 

  15. Chiang, M.; Chang, S.Y.; Whang, C.Electrophoresis 2001,22, 123–127.

    Article  CAS  Google Scholar 

  16. Nishi, H.; Nakamura, K.; Nakai, H.; Sato, T.J. Chromatogr. A 1997,757, 225–235.

    Article  CAS  Google Scholar 

  17. Okafo, G.N.; Camilleri, P.J. Microbol. Sep. 1993,5, 149–153.

    Article  CAS  Google Scholar 

  18. Roach, M.C.; Harmony, M.D.Anal. Chem. 1987,59, 411–415.

    Article  CAS  Google Scholar 

  19. Collins, G.E.; Rose-Pehrsson, S.L.Analyst 1994,119, 1907–1913.

    Article  Google Scholar 

  20. Zhao, J.; Chen, D.; Dovichi, N.J.J. Chromatogr. 1992,608, 117–120.

    Article  CAS  Google Scholar 

  21. Aminuddin, M.; Miller, J.N.Talanta 1995,42, 775–778.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Applied Chemistry, Chaoyang University of Technology, 413, Taiwan, Wufeng, Taichung County

    S. Chang & H. Yang

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  1. S. Chang
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  2. H. Yang
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Chang, S., Yang, H. Determination of baclofen by derivatization with anthracene-2,3-dicarboxaldehyde followed by capillary electrophoresis with laser-induced fluorescence detection. Chromatographia 57, 825–829 (2003). https://doi.org/10.1007/BF02491772

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  • DOI: https://doi.org/10.1007/BF02491772

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