Summary
Capillary electrochromatography has been performed with a cinnamic acid polymer-based monolithic stationary phase. The reproducibility and stability of the column in buffers of very extreme pH were found to be satisfactory. The effects on electroosmotic flow of operating conditions such as applied voltage, salt concentration, pH, and mobile phase organic modifier content were investigated. The retention mechanism of small neutral compounds on such a column was shown to be similar to that in reversed-phase high-performance liquid chromatography.
References
Peters, E.C.; Petro, M.; Svec, F.; Fréchet, J.M.J. Anal. Chem. 1997,69, 3646–3649.
Peters, E.C.; Petro, M.; Svec, F.; Fréchet, J.M.J. Anal. Chem. 1998,70, 2288–2295.
Peters, E.C.; Petro, M.; Svec, F.; Fréchet, J.M.J. Anal. Chem. 1998,70, 2296–2302.
Yu, C.; Svec, F.; Fréchet, J.M.J. Electrophoresis 2000,21(1), 120–127.
Koide, T.; Ueno, K.J. Chromatogr. A 2000,893, 177–187.
Koide, T.; Ueno, K.Anal. Sci. 1999,15, 791–794.
Koide, T.; Ueno, K.Anal. Sci. 1998,14, 1021–1023.
Koide, T.; Ueno, K.J. Chromatogr. A 2001,909, 305–315.
Jiang, J.; Jiskra, J.; Claessens, H.A.; Cramers, C.A.J. Chromatogr. A 2001,923, 215–227.
Bedair, M.; Rassi, Z.E.Electrophoresis 2002,23(17), 2938–2948.
Yan, W.-Y.; Zhang, Z.-C.; Gao, R.-Y.; Yan, C.; Wang, Q.-S.J. Liq. Chromatogr. Related Technol. 2002,25, 2963–2974.
Ngola, S.M.; Fintschenko, Y.; Choi, W.-Y.; Shepodd, T.J.Anal. Chem. 2001,73, 849–856.
Shediac, R.; Ngola, S.M.; Throckmorton, D.J.; Anex, D.S.; Shepodd, T.J.; Singh, A.K.J. Chromatogr. A 2001,925, 251–263.
Koide, T.; Ueno, K.Anal. Sci. 2000,16, 1065–1070.
Hoegger, D.; Freitag, R.J. Chromatogr. A 2001,914, 211–222.
Palm, A.; Novotny, M.V.Anal. Chem. 1997,69, 4499–4507.
Que, A.H.; Konse, T.; Baker, A.G.; Novotny, M.V.Anal. Chem. 2000,72, 2703–2710.
Zhang, M.; Rassi, Z.E.Electrophoresis 2001,22(12), 2593–2599.
Xiong, B.; Zhang, L.; Zhang, Y.; Zou, H.; Wang, J.J. High Resol. Chromatogr. 2000 23(1), 67–72.
Ericson, C.; Hjertén, S.Anal. Chem. 1999,71, 1621–1627.
Koide, T.; Ueno, K.J. High Resol. Chromatogr. 2000,23(1), 59–66.
Wu, R.; Zou, H.; Ye, M.; Lei, Z.; Ni, J.Electrophoresis 2001,22(3), 544–551.
Hjertén, S.J. Chromatogr. A 1985,347, 191–195.
Cahours, X.; Morin, Ph.; Dreux, M.J. Chromatogr. A 1999,845, 203–216.
Choudhary, G.; Horváth, C.J. Chromatogr. A 1997,781, 161–183.
Meites, L.Handbook of Analytical Chemistry, 2nd edn, McGraw-Hill, New York,1963.
Lin, B.; Zhou, X.; Han P.Applications of High Performance Liquid Chromatography in Life Science, Shandong Science and Technology Press, Jinan, China,1996.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yan, W., Gao, R., Zhang, Z. et al. Cinnamic acid polymer-based monolithic column for capillary electrochromatography. Chromatographia 57, 819–823 (2003). https://doi.org/10.1007/BF02491771
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02491771