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Macrocycleversus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes

Chromatographia volume 57, pages 757–765 (2003)Cite this article

Summary

Potentiometric detection of lipophilic drugs such as clenbuterol, ambroxol and bromhexine in standard-bore cyano and narrow-bore hybrid, reversed-phase HPLC systems was developed to enable specific determination of these mucolytic agents in some marketed oral-formulations. The macrocycle and podant-type, neutral ionophores, respectively: hexakis(2,3,6-tri-O-octyl)-α-cyclodextrin (CDX) andN,N,N′,N′-tetracyclohexyl-oxybis(o-phenyleneoxy)diacetamide (TOPA), were used to construct PVC-based, liquid membrane electrodes. In acidic aqueous-acetonitrile, mobile phases both ionophores improved the sensitivity of these electrodes for mucolytic agents when compared to single-wavelength UV detection—as well as with the ionophore-free based electrode. In the case of bromhexine, indicating highest lipophilicity (logP6.40), detection limits of 2.6×1010 and 1.4×1010M (injected concentration) were achieved with a signal-to-noise (S/N) ratio of 3 with the TOPA-modified, liquid membrane electrode in the various RP-HPLC systems. The results of procedures validated for simultaneous potentiometric determination of clenbuterol and ambroxol in tablets marketed in different countries are also presented.

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Authors and Affiliations

  1. Department of Drug Chemistry, Faculty of Pharmacy, Ludwik Rydygier Medical University, Jagiellonska 13, 85-067, Bydgoszcz, Poland

    G. Bazylak & L. J. Nagels

  2. Department of Chemistry, Faculty of Science & Biomedicine, University of Antwerpen RUCA, Groenenborgerlaan 171, 2020, Antwerpen, Belgium

    G. Bazylak

  3. INQUIMAE, Facultad de Ciencias Exactas & Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, C1428EHA, Buenos Aires, Argentina

    M. E. Monge

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  1. G. Bazylak
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  3. M. E. Monge
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Bazylak, G., Nagels, L.J. & Monge, M.E. Macrocycleversus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes. Chromatographia 57, 757–765 (2003). https://doi.org/10.1007/BF02491762

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  • DOI: https://doi.org/10.1007/BF02491762

Key Words

  • Column liquid chromatography
  • Potentiometric detection
  • Lipophilic drugs
  • Pharmaceutical formulations