Chromatographia

, Volume 57, Issue 9–10, pp 629–633 | Cite as

Rapid separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic stationary phase

  • Guichen Ping
  • Weibing Zhang
  • Lihua Zhang
  • P. Schmitt-Kopplin
  • Yukui Zhang
  • A. Kettrup
Originals Electrochromatography/Electrophoresis

Summary

Separation of nucleosides by capillary electrochromatography with a methacrylate-based monolithic column is described. Because the nucleosides are relatively hydrophilic compounds, chromatographic parition is very weak on non-polar stationary phases. A small quantity of organic modifier is usually required to increase their retention factars but, because the wettability of the stationary phase by the mobile phase is rather poor, bubble formation can be a significant problem. This situation was improved by use of a polymeric monolithic column with higher level charged monomer in the polymerization mixlure, because of the presence of charged moieties on the surface of the monolithic stationary phase. The very high stability under conditions of very low organic modifier concentration used in the experiment showed that the wettability of the stationary phase by the mobile phase was satisfactory. The effect of pH, orgonic modifier content, and applied voltage on the separation was investigated. Baseline separction of six nucleosides was achieved within 4.5 min on this type of column. Because of the presence of charged moieties and butyl groups on the surface of the monolithic column, the mechanism of separation might involve both ion-exchange and hydrophobic interactions.

Key words

Capillary electrochromatography Monolithic column Nucleosides 

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References

  1. [1]
    Helboe, T.; Hansen, S.H.J. Chromatogr. A 1999,836, 315, 324.CrossRefGoogle Scholar
  2. [2]
    Liebich, H.M.; Stefano, C.D.; Wixforth, A.; Schmid, H.R.J. Chromatogr. A 1997,763, 193 197.CrossRefGoogle Scholar
  3. [3]
    Simek, P.; Jegorov, A.; Dusbábek F.J. Chromatogr. A 1994,679, 195 200.CrossRefGoogle Scholar
  4. [4]
    Qurishi, R.; Kaulich, M.; Müller, C.E.J. Chromatogr. A 2002,952, 275 281.CrossRefGoogle Scholar
  5. [5]
    Liu, J.; Banks, F.; Novotny, J.,J. Microcol. Sep. 1989,1, 136, 141.CrossRefGoogle Scholar
  6. [6]
    Minakuchi, H.; Ishizuka, N. Nakanishi, K.; Soga, N.; Tanaka, N.J. Chromatogr. A 1998,828, 83, 90.CrossRefGoogle Scholar
  7. [7]
    Jiang, T.; Jiskra, J.; Claessens, H. A.; Cramers, C.A.J. Chromatogr. A 2001,923, 215, 227.CrossRefGoogle Scholar
  8. [8]
    Ericson, C.; Liao, J. L.; Nakazato, K.; Hjertén, S.J. Chromatogr. A 1997,767, 33 41.CrossRefGoogle Scholar
  9. [9]
    Xiong, B.H.; Zhang, L.H.; Zhang, Y.K.; Zou, H.F.; Wang, J.D.J. High Resol. Chromatogr. 2000,23, 67 72.CrossRefGoogle Scholar
  10. [10]
    Sykora, D.; Svec, F.; Fréchet, J.M.J.J. Chromatogr. A 1999,852, 297 304.CrossRefGoogle Scholar
  11. [11]
    Svec, F.; Fréchet, J.M.J.,J. Chromatogr. 1995,702, 89 95.CrossRefGoogle Scholar
  12. [12]
    Fujimoto C.; Fujise, Y.; Matsuzawa, E.Anal. Chem. 1996,68, 2753 2757.CrossRefGoogle Scholar
  13. [13]
    Peters, E.C.; Petro, M.; Svec, F.; Fréchet, J.M.J.Anal. Chem. 1998,70, 2296 2302.CrossRefGoogle Scholar
  14. [14]
    Fujimoto, C.; Kino, J.; Sawada, H.J. Chromatogr. A 1995,716, 107 113.CrossRefGoogle Scholar
  15. [15]
    Zhang, M.Q.; Rassi, Z.E.Electrophoresis 1999,20, 31 36.CrossRefGoogle Scholar
  16. [16]
    Cahours, X.; Morin, P.; Agrofoglio, L.; Dreux, M.J. High Resol. Chromatogr. 2000,23, 138 142.CrossRefGoogle Scholar
  17. [17]
    Peters, E.C.; Petro, M.; Svec, F.; Frechét, J.M.J.Anal. Chem. 1998,70, 2288 2295.CrossRefGoogle Scholar
  18. [18]
    Peters, E.C.; Petro, M.; Svec, F.; Frechét, J.M.J.Anal. Chem. 1998,70, 2296 2302.CrossRefGoogle Scholar
  19. [19]
    Ping, G.C.; Schmitt-Kopplin, P.; Zhang, W.B.; Zhang, Y.K. Kettrup, A.Electrophoresis, accepted for publication.Google Scholar
  20. [20]
    Iberer, G.; Hahn, R.; Jungbauer, A.LC-GC,1999,17, 998 1005.Google Scholar
  21. [21]
    Ping, G.C.; Zhang, Y.K.; Zhang W.B.; Zhang, L.H.; Schmitt-Kopplin, P.; Kettrup, A.,J. Chromatogr. A, submitted for publication.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • Guichen Ping
    • 1
  • Weibing Zhang
    • 1
  • Lihua Zhang
    • 1
  • P. Schmitt-Kopplin
    • 2
  • Yukui Zhang
    • 1
  • A. Kettrup
    • 2
  1. 1.National Chromatographic Research and Analysis Center, Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianP.R. China
  2. 2.Institute for Ecological ChemistryGSF-National Center for Environment and HealthNeuherbergGermany

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