Advertisement

Chromatographia

, Volume 57, Issue 9–10, pp 581–592 | Cite as

Food supplements ofTribulus terrestris L.: An HPLC-ESI-MS method for an estimation of the saponin content

  • N. Mulinacci
  • P. Vignolini
  • G. la Marca
  • G. Pieraccini
  • M. Innocenti
  • F. F. Vincieri
Originals Column Liquid Chromatography

Summary

An HPLC-ESI-MS method in a positive mode is proposed to qualitatively analyse the saponin fractions from dietary supplements ofTribulus terrestris L. This method allows for theseparation of structural isomers, as highlighted for some terrestrosines. The MS fragmentation pattern resulted diagnostic to collect structural information and the characteristic loss of water from some furostanolic saponins was highlighted for the first time. Due to the wide number of glycosidic furostanolic and spirostanolic saponins extracted from tribulus, a complete reference “molecular weight map” was created. Finally, a seml-quantitative evaluation of the total saponin content was carried cut by HPLC-MS using protodioscin as external standard.

Key Words

Column liquid chromatography ESI-MS Saponins Extrelut cartidge extraction Tribulus terrestris 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Fang, S.P.; Hao, C.Y.; Sun, W.; Liu, Z.Q.; Liu S.Y.Rapid Commun Mass Spectrom. 1998,12, 589–594.CrossRefGoogle Scholar
  2. [2]
    Xu, Y.X.; Chen, H.S.; Liang, H.Q.; Gu, Z.B.; Liu, W.Y.; Leung, W.N.; Li, T.J.Planta Med. 2000,66, 545–550.CrossRefGoogle Scholar
  3. [3]
    Wu, G.; Jiang, S.; Jiang, F.; Zhu, D.; Wu, H.; Jiang, S.Phytochemistry 1996,42(6), 1677–1688.CrossRefGoogle Scholar
  4. [4]
    Yan, W.; Ohtani, K.; Kasai, R.; Yamasak, K.Phytochemistry 1996,42(5), 1417–1422.CrossRefGoogle Scholar
  5. [5]
    Fang, S.P.; Hao, C.Y.; Liu, Z.Q.; Song, F.R.; Liu, S.Y.Planta Med. 1999,65(1), 68–73.CrossRefGoogle Scholar
  6. [6]
    Wang, Y.; Ohtani, K.; Kasai, R.; Yamasaki, K.Phytochemistry 1997,45(4), 811–817.CrossRefGoogle Scholar
  7. [7]
    Bedir, E.; Khan, L.A.J. Nat. Prod. 63 2000, 1699–1701CrossRefGoogle Scholar
  8. [8]
    Cai, L.; Wu, Y.; Zhang, J.; Pei, F.; Xu, Y.; Xie, S.; Xu, D.Planta Med 2001,67 (2), 196–198.CrossRefGoogle Scholar
  9. [9]
    Xu, Y.X.; Chen, H.S.; Liu, W.Y.; Gu, Z.B.; Liang, H.Q.Phytochemistry 1998,49(1), 199–201.CrossRefGoogle Scholar
  10. [10]
    Kostova, I.; Dinchev, D.; Rentsch, G.H.; Dimitrov, V.; Ivanova, A.Z.Naturforsch 2002,57(1–2), 33–38.Google Scholar
  11. [11]
    Olesezek, W.A.J. Chromatogr. A. 2002 967(1), 147–162.CrossRefGoogle Scholar
  12. [12]
    Ganzera, M.; Bedir, E.; Khan, I.A.J. Pharm Sci. 2001,90(11), 1752.CrossRefGoogle Scholar
  13. [13]
    Bourke, C.A.; Stevens, G.R.; Carrigan, M.J.Australian Veterinary Journal 1992,69(7), 163–165.Google Scholar
  14. [14]
    Tian-Shung, W.; Li-Shia, S.; Shang-Chu, K.Phytochemistry 1999,50 (8), 1411–1415.CrossRefGoogle Scholar
  15. [15]
    Li, J.X.; Shi, Q.; Xiong Q.B.; Prasain, J.K.; Tezuka, Y.; Hareyama, T.; Wang, Z.T.; Tanaka, K.; Namba, T.; Kadota, S.Planta Med. 1998,64(7), 628–631.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • N. Mulinacci
    • 2
  • P. Vignolini
    • 2
  • G. la Marca
    • 1
  • G. Pieraccini
    • 1
  • M. Innocenti
    • 2
  • F. F. Vincieri
    • 2
  1. 1.Dipartimento di Scienze FarmaceuticheUniversità degli Studi di FirenzeFirenzeItaly
  2. 2.Centro Interdipartimentale di Spettrometria di MassaUniversità degli Studi di FirenzeFirenzeItaly

Personalised recommendations