, Volume 57, Issue 9–10, pp 581–592 | Cite as

Food supplements ofTribulus terrestris L.: An HPLC-ESI-MS method for an estimation of the saponin content

  • N. Mulinacci
  • P. Vignolini
  • G. la Marca
  • G. Pieraccini
  • M. Innocenti
  • F. F. Vincieri
Originals Column Liquid Chromatography


An HPLC-ESI-MS method in a positive mode is proposed to qualitatively analyse the saponin fractions from dietary supplements ofTribulus terrestris L. This method allows for theseparation of structural isomers, as highlighted for some terrestrosines. The MS fragmentation pattern resulted diagnostic to collect structural information and the characteristic loss of water from some furostanolic saponins was highlighted for the first time. Due to the wide number of glycosidic furostanolic and spirostanolic saponins extracted from tribulus, a complete reference “molecular weight map” was created. Finally, a seml-quantitative evaluation of the total saponin content was carried cut by HPLC-MS using protodioscin as external standard.

Key Words

Column liquid chromatography ESI-MS Saponins Extrelut cartidge extraction Tribulus terrestris 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • N. Mulinacci
    • 2
  • P. Vignolini
    • 2
  • G. la Marca
    • 1
  • G. Pieraccini
    • 1
  • M. Innocenti
    • 2
  • F. F. Vincieri
    • 2
  1. 1.Dipartimento di Scienze FarmaceuticheUniversità degli Studi di FirenzeFirenzeItaly
  2. 2.Centro Interdipartimentale di Spettrometria di MassaUniversità degli Studi di FirenzeFirenzeItaly

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