, Volume 57, Issue 3–4, pp 255–260 | Cite as

First mass spectrometric detection of boldenone in horse mane samples

  • M. A. Popot
  • N. Stojiljkovic
  • P. Garcia
  • Y. Bonnaire
  • J. C. Tabet
Short Communications Gas Chromatography


In order to investigate the detection of boldenone in horse mane samples, a boldenone study was conducted on two horses. The analytical procedure consisted in a hydrolysis using the Sorensen buffer, a liquid-liquid extraction using diethyl ether and a PFPA derivatization. The instrumental method was a gas chromatography sequential mass spectrometry performed on an ion trap instrument in full scan mode. The limit of detection was estimated to 1 pg mg−1. The detection of boldenone in the mane was made possible for up to 12 months after administration.

Key Words

Gas chromatography—mass spectrometry Horse hair Boldenone 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Wheeler, M.J.; Zhong, Y.B.; Kieman, A.T.; Coutts, S.B.J. Endocrinology 1998,159, R5-R8.CrossRefGoogle Scholar
  2. [2]
    Scherer, C.; Wachter, U.; Wudy, S.A.Analyst 1998,123, 2661–2663.CrossRefGoogle Scholar
  3. [3]
    Whittem, T.; Davis, C.; Beresford, G.D.; Gourdie T.Aust. Vet J. 1998,76, 6, 426–427.Google Scholar
  4. [4]
    Jouvel, C.; Maciejewski, P.; Garcia, P.; Bonnaire, Y.; Horning, S.; Popot, M.A.Analyst 2000,10, 1765–1769.CrossRefGoogle Scholar
  5. [5]
    Popot, M.A.; Boyer, S.; Maciejewski, P.; Garcia, P.; Bonnaire, Y.; Beyet, L.; Lesage, D.; Tabet, J.C.Chromatographia Supplement2001,53, 375–379.Google Scholar
  6. [6]
    Bonnaire, Y.; Dehennin, L.; Plou, P.; Popot, M.A.; Toutain, P.L. InProceedings of the 10th International Conference of Racing Analysts and Veterinarians Stockolm, Kallings, P.; Bondesson, U.; Houghton, E. Eds, R & W Publications, Newmarket,1994, 187–194.Google Scholar
  7. [7]
    Dumasia, M.C.; Houghton, E.; Bradley, C.V.; Williams, D.H.Biol. Mass Spectrom. 1983,10, 7, 434–440.CrossRefGoogle Scholar
  8. [8]
    Shänzer, W.; Donike, M.Biol Mass Spectrom. 1992,21, 3–16.CrossRefGoogle Scholar
  9. [9]
    Van Puymbroeck, M.; Kuilman, M.E.M.; Maas, R.F.M.; Witkamp, R.F.; Leyssens, L.; Vanderzande, D.; Gelan, J.; Raus, J.Analyst 1998,123, 2681–2686.CrossRefGoogle Scholar
  10. [10]
    Popot, M.A.; Boyer, S.; Maciejewski, P.; Garcia, P.; Dehennin, L.; Bonnaire Y. InProceedings of the 13th International Conference of Racing Analysts and Veterinarians, Cambridge, Williams, R.B.; Houghton E.; Wade, J.F. Eds, R & W Publications, Newmarket,2000, 115–120.Google Scholar
  11. [11]
    Choi, M.H.; Kim, J.Y.; Chung, B.C.Analytical letters 1999,32, 7, 1313–1322.Google Scholar
  12. [12]
    Kintz, P.; Cirimele, V.; Jeanneau, T.; Ludes, B.J. Anal. Toxicol. 1999,23, 352–356.Google Scholar
  13. [13]
    Gaillard, Y.; Vayssette, F.; Balland, A.; Pepin, G.J. Chromatogr. B1999,735, 189–205.Google Scholar
  14. [14]
    Schänzer, W.; Geyer, H.; Gotzman, A.; Horning, S.; Marek-Engelke, U.; Nitschke, R. InProceeding of the 12 th.Cologne workshop, Cologne, Shänzer. W. Ed.,1994, 211.Google Scholar
  15. [15]
    Segura, J.; Pichini, S.; Peng, S.H.; de la Tore, X.Forensic Sci. Int. 2000,107, 347–359.CrossRefGoogle Scholar
  16. [16]
    Höld, K.; Borges, C.R.; Wilkins, D.G.; Rollins, D.E.; Joseph Jr. R.E.J. Anal. Toxicol. 1999,23, 416–423.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • M. A. Popot
    • 1
  • N. Stojiljkovic
    • 1
  • P. Garcia
    • 1
  • Y. Bonnaire
    • 1
  • J. C. Tabet
    • 2
  1. 1.LAB/FNCFChâtenay-MalabryFrance
  2. 2.L.C.O.B, U.M.R 7613Paris Cedex 05France

Personalised recommendations