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An on-line solid phase extraction—Liquid chromatography—Tandem mass spectrometry method for the analysis of citalopram, fluvoxamine, and paroxetine in human plasma

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Summary

A specific and sensitive direct-injection high performance liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry (HPLC-APCI-MS-MS) method has been developed for the rapid identification and quantitative determination of citalopram, fluvoxamine, and paroxetine in human plasma. After dilution with 0.1% formic acid, plasma samples were injected into the LC-MS-MS system. Proteins and other large biomolecules were removed during an on-line sample cleanup step. The inter- and intra-assay coefficients of variation for all compounds were <11%. The total analysis time was 6 min per sample. The proposed method permits direct analysis of plasma samples without time-consuming sample preparation.

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Authors and Affiliations

  1. Institute of Forensic Medicine, Karl-Franzens University Graz, Universitaetsplatz 4/II, 8010, Graz, Austria

    M. Kollroser

  2. Institute of Molecular Biology, Biochemistry, and Microbiology (IMBM), Karl-Franzens University Graz, Heinrichstrasse 31 a, 8010, Graz, Austria

    C. Schober

Authors
  1. M. Kollroser
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  2. C. Schober
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Kollroser, M., Schober, C. An on-line solid phase extraction—Liquid chromatography—Tandem mass spectrometry method for the analysis of citalopram, fluvoxamine, and paroxetine in human plasma. Chromatographia 57, 133–138 (2003). https://doi.org/10.1007/BF02491705

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  • DOI: https://doi.org/10.1007/BF02491705

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