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HPLC validation of ameziniummetilsulfate in human plasma from uremia patient treated by dialysis

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Summary

A high-performance liquid chromatography (HPLC) method using an internal standard is validated for the determination of 4-amino-6-methoxy-1-phenyl-pyridazinium methyl sulfate (ameziniummetilsulfate, AM) in a uremia patient treated by artificial dialysis. The method involves liquid-liquid extraction and ion-supression, reverse-phase HPLC on an end-capped C-18 column. There is no interference by plasma components or AM metabolites in the HPLC analysis. Average recovery rate using liquid-liquid extraction was 88.7% and limit of determination was 2 ng mL−1. This concentration was sufficient to determine AM in human plasma. Accuracy and precision was <5.6% and 16.2%, respectively, at 2 ng mL−1, the limit of determination.

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Authors and Affiliations

  1. National Institute of Health Sciences, Ministry & Welfare, 1-18-1, Kamiyoga, Setagaya, 158, Tokyo, Japan

    H. Shintani

  2. Health care facility of Namiki hospital, 2-10-22, Uchidabashi, Minamiku, Nagoya, Japan

    F. Hayashi

Authors
  1. H. Shintani
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  2. F. Hayashi
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Shintani, H., Hayashi, F. HPLC validation of ameziniummetilsulfate in human plasma from uremia patient treated by dialysis. Chromatographia 53, 574–578 (2001). https://doi.org/10.1007/BF02491626

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  • DOI: https://doi.org/10.1007/BF02491626

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