Summary
A separation method for Bt toxic protein CrylAb from Bt transgenic rice (Ke Ming Dao) by ion-exchange chromatography has been developed and validated. Bt transgenic rice-extract containing CrylAb toxic protein is the matrix for separation. The method has a good reproducibility and is easy to operate. It was shown that the Bt fraction isolated by chromatography had high insecticidal activity.
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Wu, Z.P., Xu, B.J. & Chen, Z.Y. Separation and purification ofBacillus thuringiensis(Bt) toxic protein CrylAb from transgenic rice by ion-exchange chromatography. Chromatographia 53, 571–573 (2001). https://doi.org/10.1007/BF02491625
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DOI: https://doi.org/10.1007/BF02491625