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Modulation of chromatographic performances of HSA-based HPLC column by reversible binding of lithocholic acid

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Summary

The performance of a HSA-based HPLC column has been improved by modulating the binding properties of the anchored protein using lithocholic acid as a selective modifier in the mobile phase. In particular, retention decreased for both drugs binding to sites I or II, while the enantioselectivity was maintained for most analytes. The advantages of using lithocholic acid in the mobile phase were impressive in the case of profens, for which a considerable reduction in analysis time and a significant increase in enantioselectivity were obtained. The results were rationalized by considering lithocholic acid as a direct competitor for site II binding drugs and indirect competitor for site I binding drugs.

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References

  1. Domenici, E.; Bertucci, C.; Salvadori, P.; Felix, G.; Cahagne, I.; Motellier, S.; Wainer, I.W.Chromatographia 1990,29, 170–176.

    Article  CAS  Google Scholar 

  2. Noctor, T.A.G. Felix, G.; Wainer, I.W.Chromatographia 1991,31, 55–59.

    Article  CAS  Google Scholar 

  3. Chosson, E.; Uzan, S.; Gimenez, F.; Wainer, I.W.; Farinotti, R.Chirality 1993,5, 71–77.

    Article  CAS  Google Scholar 

  4. Loun, B.; Hage, D.S.Anal. Chem. 1994,66, 3814–3822.

    Article  CAS  Google Scholar 

  5. I. Fitos, J. Visy, M. Simonyi, J. Hermansson,Chirality 1999,11, 115–120.

    Article  CAS  Google Scholar 

  6. Domenici, E.; Bertucci, C.; Salvadori, P.; Motellier, S.; Wainer, I.W.Chirality 1990,2, 263–268.

    Article  CAS  Google Scholar 

  7. Hage, D.S.; Noctor, T.A.G.; Wainer, I.W.J. Chromatogr. A 1995,693, 23–32.

    Article  CAS  Google Scholar 

  8. Oravcová, J.; Böhs, B.; Lindner, W.J. Chromatogr. B Biomed Appl 1996,677, 1–28.

    Article  Google Scholar 

  9. Yang, J.; Hage, D.S.J. Chromatogr. 1993,645, 241–250.

    Article  CAS  Google Scholar 

  10. Bertucci, C.; Canepa, A.; Ascoli, G.; Lopes Guimaraes, L.F.; Felix, G.Chirality 1999,11, 675–679.

    Article  CAS  Google Scholar 

  11. Kaliszan, R.; Noctor, T.A.G., Wainer, I.W.Chromatographia 1992,33, 546–550.

    Article  CAS  Google Scholar 

  12. Noctor, T.A.G.; Pham, C.D.; Kaliszan, R.; Wainer, I.W.Mol. Pharmacol. 1992,42, 506–511.

    CAS  Google Scholar 

  13. Kaliszan, R.; Noctor, T.A.G.; Wainer, I.W.Mol. Pharmacol. 1992,42, 512–517.

    CAS  Google Scholar 

  14. Andrisano, V.; Bertucci, C.; Cavrini, V.; Recanatini, M.; Cavalli, A.; Varoli, L.; Felix, G.; Wainer, I.W.J. Chromat. A 2000,876, 75–86.

    Article  CAS  Google Scholar 

  15. Noctor, T.A.G.; Wainer, I.W.J. Liquid Chromatogr. 1993,16, 783–800.

    CAS  Google Scholar 

  16. Ascoli, G.; Bertucci, C.; Salvadori, P.Biomed. Chromat. 1998,12, 248–254.

    Article  CAS  Google Scholar 

  17. Noctor, T.A.G.; Wainer, I.W.; Hage, D.S.J. Chromatogr. 1992,577, 305–315.

    CAS  Google Scholar 

  18. Kragh-Hansen, U.Dan. Med. Bull. 1990,37, 54–84.

    Google Scholar 

  19. Sudlow, G.; Birkett, D.J.; Wade, D.N.Mol. Pharmacol. 1975,12, 1052–1061

    Google Scholar 

  20. Bell, S.C.; McCaully, R.J.; Gochman, C.; Childress, S.J.; Gluckman, M.I.J. Med. Chem. 1968,11, 457.

    Article  CAS  Google Scholar 

  21. Roda, A.; Cappelleri, G.; Aldini, R.; Roda, E.; Barbara, L.,J. Lipid Res. 1982,23, 490–495.

    CAS  Google Scholar 

  22. Scagnolari, S.; Roda, A.; Fini, A.; Grigolo, B.Biochim. Biophys. Acta 1984,791, 274–277.

    CAS  Google Scholar 

  23. Takikawa, H.; Sugiyama, Y.; Hanano, M.; Kurita, M.; Yoshida, H.; Sugimoto, T.Biochim. Biophys. Acta 1987,926, 145–153.

    CAS  Google Scholar 

  24. Takikawa, H.; Sekiya, Y.; Yamanaka, M.; Sugiyama, Y.Biochim. Biophys. Acta 1995,1244, 277–282.

    Google Scholar 

  25. Goto, S.; Watanabe, S.; Maehara, S.; Takata, Y.; Tateyama, T.J. Pharmacobio-Dyn. 1985,8, 440–447.

    CAS  Google Scholar 

  26. Bowmer, C.J.; Donoghue, P.G.; Leong, C.F.; Yates, M.S.J. Pharm. Pharmacol.,1985,37, 812–815.

    CAS  Google Scholar 

  27. Bertucci, C.; Wainer, I.W.Chirality 1997,9, 335–340.

    Article  CAS  Google Scholar 

  28. Alebic-Kolbah, T.; Kajfez, F.; Rendic, S.; Sunjic, V.; Konowal, A.; Snatzke, G.Biochem. Pharmacol. 1979,28, 2457–2464.

    Article  CAS  Google Scholar 

  29. Bertucci, C.; Domenici, E.; Salvadori, P.Chirality 1990,2, 167–174.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Dipartimento di Scienze Farmaceutiche, Università di Bologna, via Belmeloro 6, 40126, Bologna, Italy

    C. Bertucci, V. Andrisano, R. Gotti & V. Cavrini

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  1. C. Bertucci
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  2. V. Andrisano
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  3. R. Gotti
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  4. V. Cavrini
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Bertucci, C., Andrisano, V., Gotti, R. et al. Modulation of chromatographic performances of HSA-based HPLC column by reversible binding of lithocholic acid. Chromatographia 53, 515–518 (2001). https://doi.org/10.1007/BF02491614

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  • DOI: https://doi.org/10.1007/BF02491614

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