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Application of restricted-access media column in coupled-column RPLC with UV detection and electrospray mass spectrometry for determination of azole pesticides in urine

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Summary

Triadimenol, triadimefon and tetraconazole were simultaneously determined in human urine by coupled-column liquid chromatography with both UV and ES-MS detection. These procedures were validated and the values of some merit figures, such as, linear range, detection limits, precision and recovery, obtained with both methods, are compared. A 5 μm GFF-II, internal-surface, reversed-phase (ISRP, Pinkerton) column coupled to a 3 μm reversed-phase, Microsphere C18 column were used. To increase sensitivity a solid-phase extraction step with C18 cartridge was carried out yielding recoveries 80–107% (n=5) with RSD 3–8% for the LC-LC-UV method and 88–108% (n=5), RSD 5–11% for the LC-ES-MS method. Limits of detection were 1.5–3.5 μg L−1 for LC-LC-UV and 0.5–1.0 μg L−1 for LC-ES-MS. The methods were employed to detect and quantify pesticides in urine of pest control operators and non-occupationally exposed subjects.

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Almería, 04120, Almería, Spain

    J. Martínez Fernández, J. L. Martínez Vidal, P. Parrilla Vázquez & A. Garrido Frenich

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  1. J. Martínez Fernández
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  2. J. L. Martínez Vidal
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  3. P. Parrilla Vázquez
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  4. A. Garrido Frenich
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Martínez Fernández, J., Martínez Vidal, J.L., Parrilla Vázquez, P. et al. Application of restricted-access media column in coupled-column RPLC with UV detection and electrospray mass spectrometry for determination of azole pesticides in urine. Chromatographia 53, 503–509 (2001). https://doi.org/10.1007/BF02491612

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  • DOI: https://doi.org/10.1007/BF02491612

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