Summary
An inverse supercritical fluid extraction (SFE) procedure has been developed for the efficient isolation of six of the most common xanthene dyes (fluorescein; 4′,5′-dibromofluorescein; 2′,4′,5′,7′-tetrabromofluorescein; 2′,4′,5′,7′-tetrabromo-4,5,6,7-tetrachlorofluorescein; 4′,5′-diiodofluorescein and 2′,4′,5′,7′-tetraiodofluorescein) from lipstick matrices. A rapid high-performance liquid chromatographic method is also presented for the separation of the six dyes on a cyanopropyl-bonded silica column eluted with acetonitrile-methanol-acetate buffer. Extraction of the lipstick with supercritical CO2 at 350 atm and 40°C for 10 min exclusively removed the matrix components, whereas the xanthene colorants remained in the extraction vessel. After treatment with CO2, the target analytes were quantitatively recovered (>96.8%) by dispersion of the sample in ethanol under sonication. The inverse SFE method was compared with conventional liquid-liquid extraction on commercial lipstick products. The two methods produced comparable recoveries. However, inverse SFE was superior in terms of precision and speed of analysis and gave cleaner extracts.
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Scalia, S., Simeoni, S. Assay of xanthene dyes in lipsticks by inverse supercritical fluid extraction and HPLC. Chromatographia 53, 490–494 (2001). https://doi.org/10.1007/BF02491609
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DOI: https://doi.org/10.1007/BF02491609