Summary
Rapid, simple and sensitive methods have been devised for simultaneous determination of lamotrigine (LTG) and carbamazepine (CBZ) in microsamples of human plasma by high-performance liquid chromatography (HPLC) and high resolution gas chromatography (HRGC); both methods have been validated and compared.
The HPLC method was more sensitive than HRGC for both drugs, LTG and CBZ. The lowest quantitation limits (LOQ) of plasma LTG concentrations were 0.1 μg.mL−1 (HPLC) and 0.5 μg.mL−1 (HRGC); for CBZ they were 0.1 μg.mL−1 (HPLC) and 0.25μg.mL−1 (HRGC).
Both methods, are sensitive enough to work with microsamples at subtherapeutic, therapeutic and toxic concentrations, being reliable and practical for routine therapeutic monitoring of LTG. The HPLC method proposed is suitable also for pharmacokinetic studies or child patient, routine therapeutic-drug monitoring where there is a need for sensitive methods and small sample sizes.
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Goa, K.; Ross, S.; Crisp, P.Drugs 1993,46, 152–176.
Jawad, S.; Richens, A.; Goodwin, G.; Yuen, W.C.Epilepsia 1989,19, 356–363.
Messenheimer, A.J.; Ramsay, R.E.; Willmore, L.J.Epilepsia 1994,35, 113–121.
Schapel, G.J.; Beran, R.G.; Vajda, F.J.E.J. Neurol Neurosurg Pschiatry 1993,56, 448–453
Besag, F.M.C.; Wallace, S.J.; Dulac, O.; Alving, J.; Spencer, S.C.; Hosking, G.J. Pediatr 1995,127, 991–997.
Reunanen, M.; Dam, M.; Yuen, A.W.C.Epilepsy Res 1996,23, 149–155.
Brodie, M.J.; Richens, A.W.C.Lancet 1995,345, 476–479.
Shorvon, S.; Stefan, H.Epilepsia 1997,38, 45–51.
Riva, R.; Albani, F.; Contin, M.; Baruzzi, A.Clin. Pharmacokinet 1996,31, 470–493.
Cohen, A.F.; Ashby, L.; Crowley, D.; Lang, G.; Peck, A.W.Br. J. Clin. Pharmacol. 1985,20, 619–629
Jawad, S.; Oxley, J.; Yeun, W.C.; Richens, A.Br. J. Clin. Pharmacol 1986,22, 191–193.
Biddelecombe, R.A.; Dean, K.L.; Smith, C.D.; Jeal, S.C.J. Pharm. Biomed. Anal. 1990,8, 691–694.
Sailstad, J.M.; Findlay, J.W.Ther. Drug Monit 1991,13, 433–442.
Shihahi, Z.H.; Oles, K.S.J. Chromatogr. B. Biomed Appl. 1996,683, 119–123.
Watelle, M.; Demedts, P.; Franck, F.; De Deyn, P.; Wauters, A.; Neels, H.Ther. Drug Monit. 1997,19, 460–464.
Hallbach, J.; Vogel, H.; Guder, W.G.Eur. J. Clin. Chem. Clin. Biochem 1997,35, 755–759.
Ramsay, R.E.; Pellock, J.M.; Garnett, W.R.; Sanchez, R.M.; Valakas, A.M.; Otto, V.Epilepsy Res 1991,10, 191–200.
Fazio, A.; Artesi, C.; Russo, M.; Trio, R.; Oteri, G.; Pisani, F.Ther. Drug Monit 1992,14, 509–512.
Sallustio, B.C.; Morris, R.G.Ther. Drug Monit. 1997,19, 688–693.
Bartoli, A.; Marchiselli, R.; Gatti, G.Ther. Drug Monit 1997,19, 100–107.
Yamashita, S.; Furuno, K.; Kawasaki, H.; Gomita, Y.; Yoshinaga, H.; Yamatogi, Y.; Ohtahara, S.J. Chromatogr. 1995,670, 354–357.
Forssblad, E.; Eriksson, A.S.; Beck, O.J.Pharm. Biomed. Anal. 1996,14, 755–758.
Hart, A.P.; Blackwell, W.; Dasgupta, A.Ther. Drug Manit. 1997,19, 431–435.
Ren, S.; Scheuer, M.L.; Zheng, W.Ther. Drug Monit. 1998,20, 209–214.
Matar, K.M.; Nicholls, P.J.; Bawazir, S.A.; al-Hassan, M.I.; Tekle, A.J. Pharm. Biomed Anal 1998,17, 525–531.
Potter, J.M.; Donnelly, A.Ther. Drug Monit. 1998,20, 652–657.
Oertel, R.; Richter, K.; Gramatte, T.; Kirch, W.J. Chromatogr. 1998,797, 203–209.
Papadoyannis, N.; Zotou, A.C.; Samanidou, V.F.J. Liq. Chromatogr. 1995,18, 2593–2609.
Fraser, A.D.; MacNeil, W.; Isner, A.F.; Camfield, P.R.Ther. Drug Monit 1995,17, 174–178.
Cooper, J.D.H.; Shearsby, N.J.; Taylor, J.E.; Sheung, C.T.C.J. Chromatogr. 1997,702, 227–233.
Angelis-Stoforidis, P.; Morgan, D.J.; O'Brien, T.J.; Vajda, F.J.J. Chromatogr. 1999,30, 113–118.
Lensmeyer, G.L.; Gidal, B.E.; Wiebe, D.A.Ther. Drug Monit. 1997,19, 292–300.
Cociglio, M.; Alric, R.; Bouvier, O.J. Chromatogr. 1991,572, 269–276.
Londero, D.; Greco, P.J. Chromatogr. 1997,691, 139–144.
Ramachandran, S.; Underhill, S.; Jones, S.R.Ther. Drugs Monit. 1994,16, 75–82.
Sinz, M.W.; Remmel, R.P.J. Chromatogr. 1991,571, 217–230.
Meyler, M.; Kelly, M.; Smyth, M.Chromatographia 1993,36, 27–32.
Blanchard, J.J. Chromatogr. 1981,226, 455–457.
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Queiroz, M.E.C., Carrilho, E., Carvalho, D. et al. Comparison of high-resolution gas chromatography and high-performance liquid chromatography for simultaneous determination of lamotrigine and carbamazepine in plasma. Chromatographia 53, 485–489 (2001). https://doi.org/10.1007/BF02491608
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DOI: https://doi.org/10.1007/BF02491608