Summary
Fast separations of perfluorinated polyethers and polymethylsiloxanes that are composed of 50–80 oligomers were demonstrated in packed capillary column supercritical fluid chromatography (SFC) using a carbon dioxide mobile phase. Separations were accomplished within 10 min using a 13 cm×250 μm i.d. column packed with 2 μm porous octadecyl bonded silica (ODS) particles. Effects of particle diameter of the packing material and pressure programming on separation were investigated, and packed column SFC was compared with open tubular column SFC. Results show that as the particle diameter was decreased from 5 to 3 to 2 μm and the column length was reduced from 85 to 43 to 13 cm, the separation time could be reduced from 70 to 20 to 10 min while still maintaining similar separation (resolution). Short columns packed with small porous particles are very suitable for fast SFC separations of polymers.
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Schmitz, F.P.; Klesper, E.J. Chromatogr. 1987,388, 3.
Pinkston, J.D.; Bowling, D.J.; Delaney, T.E.J. Chromatogr. 1989,474, 97.
White, C.M.; Houck, R.K.J. High Resol. Chromatogr. 1986,9, 4.
Petersson, P.; Markides, K.E.J. Chromatogr. A 1994,666, 381.
Wolf, C.; Pirkle, W.H.LC-GC 1997,15, 352.
Anton, K.; Eppinger, J.; Frederiksen, L.; Francotte, E.; Berger, T.A.; Wilson, W.H.J. Chromatogr. 1994,666, 395.
Widmer, H.M. in: “Modern Supercritical Fluid Chromatography”, White, C.M. Ed., Hüthig, New York,1988, Chapter 11.
Chang, H.-C.K.; Taylor, L.T.J. Chromatogr. Sci. 1990,28, 29.
Smith, R.D.; Kalinoski, H.T.; Udseth, H.R.; Wright, B.W.Anal. Chem. 1984,56, 2476.
Wright, B.W.; Smith, R.D.J. High Resol. Chromatogr. 1985,8, 8.
Smith, R.D.; Chapman, E.G.; Wright, B.W.Anal. Chem. 1984,57, 2829.
Wright, B.W.; Smith, R.D.J. High Resol. Chromatogr. 1986,9, 73.
Shen, Y.; Lee, M.L.Chromatographia 1997,45, 67.
Giorgetti, A.; Pericles, N.; Widmer, H.M.; Anton, K.; Datwyler, P.J. Chromatogr. Sci. 1989,27, 318.
Shen, Y.; Malik, A.; Li, W.; Lee, M.L.J. Chromatogr. A 1995,707, 303.
Li, W.; Ibaňez, E.; Malik, A.; Lee, M.L.J. Microcol. Sep. 1996,8, 175.
Wu, N.; Shen, Y.; Lopez-Avila, V.; Lee, M.L.J. Microcol. Sep. 1999,11, 287.
Shen, Y.; Yang, Y.J.; Lee, M.L.Anal. Chem. 1997,69, 628.
Bouigeon, C.; Thiébaut, D.; Caude, M.Anal. Chem. 1996,68, 3622.
Berger, T.A.; Blumberg, L.M.Chromatographia A 1997,758, 117.
Järemo, M.; Karlsson, L.; Jönsson, J.Å.; Mathiasson, L.Chromatographia 1994,38, 17.
Schoenmakers, P.J.J. High Resolut. Chromatogr. 1988,11, 278.
Berger, T.A.; Wilson, W.H.Anal. Chem. 1993,65, 1451.
Bartle, K.D.; Burke, M.P.; Clifford, A.A.; Davies, I.L.; Kithinji, J.P.; Raynor, M.W.; Shilstone, G.F.; Williams, A.Eur. Chromatogr. News 1988,2, 12.
Wu, N.; Medina, J.C.; Lee, M.L.,J. Chromatogr. A, in press.
Berger, T.A.Packed Column SFC, The Royal Society of Chemistry, Cambridge, UK, Chapter 3,1995.
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Wu, N., Yee, R. & Lee, M.L. Fast supercritical fluid chromatography of polymers using packed capillary columns. Chromatographia 53, 197–200 (2000). https://doi.org/10.1007/BF02491570
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DOI: https://doi.org/10.1007/BF02491570