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Separation ofAstragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection

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Summary

A new HPLC method permitted the separation of 13 triterpene lycosides isolated from differentAstragalus species within 40 min. A water/acetonitrile gradient was used as eluent and 5 μm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector, the main saponins ofA. membranaceus could be detected at levels as low as 20.0 μg·mL−1. This method facilitated distinction of differentAstragalus species as well as the analyses of market products containingA. membranaceus. The results showed variations from 0.019 to 0.184% in the total saponin content of the market products.

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Authors and Affiliations

  1. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, 38677, University, MS, USA

    M. Ganzera, E. Bedir & I. A. Khan

  2. Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey

    E. Bedir & I. Calis

  3. Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, 38677, University, MS, USA

    I. A. Khan

Authors
  1. M. Ganzera
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  2. E. Bedir
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  3. I. Calis
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  4. I. A. Khan
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Ganzera, M., Bedir, E., Calis, I. et al. Separation ofAstragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection. Chromatographia 53, 131–134 (2000). https://doi.org/10.1007/BF02491559

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  • DOI: https://doi.org/10.1007/BF02491559

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