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Development and clinical application of a polymerase chain reaction-based assay for detectingBacteroides ureolyticus

Abstract

We developed a polymerase chain reaction (PCR)-based assay for detectingBacteroides ureolyticus. No DNA fragments hybridizing the internal probe specific forB. ureolyticus were amplified from the DNAs of other bacterial species tested. This assay can detect the amount of DNA corresponding to 18 cells ofB. ureolyticus. Using this assay, we examined urethral swab specimens from men with nongonococcal urethritis (NGU) and asymptomatic men for the presence ofB. ureolyticus. WhileB. ureolyticus was detected in 6 (12.0%) of 50 asymptomatic men, this was not significantly different from the detection rate of 19.3% (22 of 114 specimens) in men with NGU. This study demonstrates that the PCR-based assay can be applied for detectingB. ureolyticus in clinical specimens and suggests that this assay might develop into a useful tool for analyzing the pathogenicity of this organism in various organs.

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References

  1. Henriksen SD. Studies in gram-negative anaerobes: gramnegative anaerobic rods with spreading colonies. Acta Pathol Microbiol Scand 1948;25:368–375.

    Article  Google Scholar 

  2. Akhtar N, Eley A, Kinghorn GR. Simple transport medium forBacteriodes ureolyticus. Eur J Clin Microbiol Infect Dis 1989;8:321–322.

    PubMed  CAS  Article  Google Scholar 

  3. Fontaine EA, Taylor-Robinson D, Hanna NF, Coufalik ED. Anaerobes in men with urethritis. Br J Venr Dis 1982; 58:321–326.

    CAS  Google Scholar 

  4. Hawkins DA, Fontaine EA, Thomas BJ, Boustouller YL, Taylor-Robinson D. The enigma of non-gonococcal urethritis: role forBacteriodes ureolyticus. Genitourin Med 1988;64:10–13.

    PubMed  CAS  Google Scholar 

  5. Fontaine EA, Taylor-Robinson D. Evaluation of liquid transport media for the isolation of anaerobic bacteria: relevance to genital tract specimens. J Infection 1981;3:360–369.

    CAS  Article  Google Scholar 

  6. Deguchi T, Yamamoto H, Tada K, Komeda H, Iwata H, Ito Y, et al. Detection ofNeisseria gonorrhoeae from male patients with urethritis by polymerase chain reaction. J Jpn Assoc Infect Dis 1992;66:555–560.

    CAS  Google Scholar 

  7. Kato N, Ito Y, Deguchi T, Kanematsu M, Ban Y, Kawada Y, et al. Isolation ofChlamydia trachomatis from patients with urethritis. J Jpn Assoc Infect Dis 1984;58:29–38.

    Google Scholar 

  8. Deguchi T, Yamamoto H, Iwata H, Yamamoto N, Ito Y, Ban Y, et al. Detection ofChlamydia trachomatis by polymerase chain reaction. J Jpn Assoc Infect Dis 1991;65: 1183–1187.

    CAS  Google Scholar 

  9. Bauwens JE, Clark AM, Loeffelholz MJ, Herman SA, Stamm WE. Diagnosis ofChlamydia trachomatis urethritis in men by polymerase chain reaction assay of firstcatch urine. J Clin Microbiol 1993;31:3013–3016.

    PubMed  CAS  Google Scholar 

  10. Chernesky M, Castriciano S, Sellors J, Stewart I, Cunningham I, Landis S, et al. Detection ofChlamydia trachomatis antigens in urine as an alternative to swabs and cultures. J Infect Dis 1990;161:124–126.

    PubMed  CAS  Google Scholar 

  11. Chernesky M, Jang D, Sellors J, Coleman P, Bodner J, Hrusovsky I, et al. Detection ofChlamydia trachomatis antigens in male urethral swabs and urines with a microparticle enzyme immunoassay. Sex Transm Dis 1995;22:55–59.

    PubMed  CAS  Article  Google Scholar 

  12. Jaschek G, Gaydos CA, Welsh LE, Quinn TC. Direct detection ofChlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay. J Clin Microbiol 1993; 31:1209–1212.

    PubMed  CAS  Google Scholar 

  13. Moncada J, Schachter J, Shafer MA, Williams E, Gourlay L, Lavin B, et al. Detection ofChlamydia trachomatis in first catch urine samples from symptomatic and asymptomatic males. Sex Trams Dis 1994;21:8–12.

    CAS  Google Scholar 

  14. Bennett KW, Eley A, Woolley PD, Duerden BI. Isolation ofBacteroides ureolyticus from the genital tract of men with and without non-gonococcal urethiritis. Eur J Clin Microbiol Infect Dis 1990;9:825–826.

    PubMed  CAS  Article  Google Scholar 

  15. Woolley PD, Kinghorn GR, Bennett KW, Eley A. Significance ofBacteroides ureolyticus in the lower genital tract. Int J STD and AIDS 1992;3:107–110.

    CAS  Google Scholar 

  16. Bennett KW, Eley A, Woolley PD. Comparison of transport media forBacteroides ureolyticus. Eur J Clin Microbiol Infect Dis 1990;9:237–238.

    PubMed  CAS  Article  Google Scholar 

  17. Eley A, Clarry T, Bennett KW. Selective and differential medium for isolation ofBacteroides ureolyticus from clinical specimens. Eur J Clin Microbiol Infect Dis 1989;8:83–84.

    PubMed  CAS  Article  Google Scholar 

  18. Bennett KW, Eley A, Woolley PD. Isolation ofBacteroides ureolyticus from the female genital tract. Eur J Clin Microbiol Infect Dis 1991;10:593–594.

    PubMed  CAS  Article  Google Scholar 

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Yasuda, M., Deguchi, T., Uno, M. et al. Development and clinical application of a polymerase chain reaction-based assay for detectingBacteroides ureolyticus . J Infect Chemother 3, 150–153 (1997). https://doi.org/10.1007/BF02491505

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  • DOI: https://doi.org/10.1007/BF02491505

Key words

  • Bacteroides ureolyticus
  • polymerase chain reaction
  • nongonococcal urethritis