Biosystematic studies of theClosterium peracerosum-strigosum-littorale complex

I. Morphological variation among the inbreeding populations and an experimental demonstration or source of the cell size variation
  • Terunobu Ichimura
  • Makoto M. Watanabe


Numerous homothallic clones of theClosterium peracerosum-strigosum-littorale complex from various localities were obtained in axenic cultures. From comparisons of morphological characters of their vegetative cells cultured under the defined standard conditions, we have concluded that frequently observed morphological variation among local populations of the complex results not merely from phenotypical modification caused by local ecological factors. On the basis of the presence or absence of a wall thickening at cell apices, the clones could be separated into 2 groups of different genetic constitution. The group without a wall thickening could be separated further into 2 subgroups on the basis of statistical analyses of cell size variation.

Clone GA-2-2 was exceptionally variable in cell size and produced remarkably deviated forms such as so-called “dwarf” or “giant” cells. Re-cloning of single cells of such deviated forms gave rise to several subclones whose mean values of cell size were distinct from that of GA-2-2, but whose qualitative characters such as the sexual morphology and the presence of a wall thickening were indistinguishable. It was observed that in clones GA-2-15 and S-10-20 which lacked a wall thickening some subclones had mean values of cell sizes distinct from that of the original clones. It was observed that in these subclones cell size changes were accompanied by nuclear size changes. The problem of cell size variation has been discussed with special regards to polyploidy and speciation in the inbreeding populations of the complex.


Vegetative Cell Morphological Group Original Clone Vegetative Character Clonal Culture 
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coefficient of variability


standard deviation


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Copyright information

© The Botanical Society of Japan 1976

Authors and Affiliations

  • Terunobu Ichimura
    • 1
    • 2
  • Makoto M. Watanabe
    • 1
    • 2
  1. 1.Institute of Applied MicrobiologyUniversity of TokyoTokyo
  2. 2.Department of Botany, Faculty of ScienceHokkaido UniversitySapporo

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