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Cell membrane isolation from plasmodium ofPhysarum polycephalum

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Abstract

Plasmodia were fractionated to isolate a cell membrane rich fraction by sucrose density-gradient centrifugation. The fractions were identified by electron microscopic observation, PTA-chromic acid staining and assays of marker enzymes, applying the methods for cell membranes of higher plants. The cell membranes were recovered on the density of 1.13 g·cm−3.

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Abbreviations

ATPase:

adenosine triphosphatase

EDTA:

ethylendiaminetetraacetic acid

GEDTA:

glycoletherdiaminetetraacetic acid

PTA:

phosphotungstic acid

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Authors and Affiliations

  1. Department of Biology, Japan Women's University, Mejirodai, 112, Tokyo

    Takako Kaneko, Keiko Karasawa, Mamiko Sato & Masako Osumi

  2. Tokyo Metropolitan Isotope Research Institute, 158, Tokyo, Fukazava

    Eiko Hosoda

Authors
  1. Takako Kaneko
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  2. Keiko Karasawa
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  3. Mamiko Sato
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  4. Masako Osumi
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  5. Eiko Hosoda
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Kaneko, T., Karasawa, K., Sato, M. et al. Cell membrane isolation from plasmodium ofPhysarum polycephalum . Bot Mag Tokyo 96, 135–138 (1983). https://doi.org/10.1007/BF02491099

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  • DOI: https://doi.org/10.1007/BF02491099

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