Development of a push-pull microdialysis sampling technique for the quantitative determination of proteins
- 109 Downloads
An on-line push-pull sampling technique has been developed for continuous analysis of proteins of molecular weight from 5.7 to 67 kDa. The characteristics of the system include gradient elution with a total cycle time of 21 min, membrane stability, unattended automatic operation, and adjustment of the sampling mode and extraction fraction (the ratio of the concentration of analyte in the dialysate to that in the sample) by varying the effective dialysis length. The push and pull flow rates were adjusted in a manner which enabled three different modes of operation. When push-pull microdialysis was compared with conventional microdialysis sampling, significantly higher extraction fractions were obtained for all five model proteins studied. The technique has been applied to the quantification of proteins in cell samples. On-line fractionation enabled complementary MS identification of the proteins present.
Key WordsColumn liquid chromatography Microdialysis sampling Push-pull Proteins
Unable to display preview. Download preview PDF.
- Anal. Chim. Acta 1999,379.Google Scholar
- Nakamura, M.; Itano, T.; Yamaguchi, F.; Mizobuchi, M.; Tokuda, M.; Matsui, H.; Hosokawa, S.K.; Ohmoto, T.; Hatase, O.Acta Med. Okayama 1990,44, 1–8.Google Scholar
- Laurell, T.; Buttler, T.Anal. Methods Instr. 1995,2, 197–201.Google Scholar