, Volume 52, Issue 5–6, pp 319–324 | Cite as

Development of a disulfiram-modified human serum albumin-based HPLC column

  • C. Bertucci
  • V. Andrisano
  • R. Gotti
  • V. Cavrini
Originals Column Liquid Chromatography


A human serum albumin (HSA)-based HPLC column has been modified in situ by disulfiram, an alcohol-deterrent drug reported to bind cys34, the only free cysteine in HSA, under physiological conditions.

The reversible and covalent binding of disulfiram was found to change the binding properties of the protein, giving rise to a new selector which performed differently from the native albumin-based stationary phase. When low concentrations of disulfiram were used as mobile phase modifier, reversible binding resulted in a cooperative allosteric effect with improved selector performance. Covalent modification resulted in markedly reduced affinity for binding of the drugs to sites I or II, while still maintaining enantioselectivity.

This study has enabled the monitoring of interactions of disulfiram with potentially coadministered drugs, and the preparation of a chiral selector with different drug affinity and enantioselectivity.

Key Words

Column liquid chromatography Human serum albumin column Disulfiram-modified phase Chiral selection 


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Copyright information

© Frieder. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • C. Bertucci
    • 1
  • V. Andrisano
    • 1
  • R. Gotti
    • 1
  • V. Cavrini
    • 1
  1. 1.Dipartimento di Scienze FarmaceuticheUniversità di BolognaBolognaItaly

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