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Rapid determination of intramacrophagic amphotericin B by direct injection HPLC

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Summary

The uptake and kinetic release of the antifungal amphotericin B by phagocytic cells appears to be important in the toxicity-activity mechanism of the drug at the cellular level. An analytical HPLC method has been developed, that is selective, sensitive and reproducible for the determination of amphotericin B uptake by J774.2 macrophage-like cells. Chromatography was on a reversed-phase, end-capped column (Lichrospher Select B C8) and samples were eluted with a mobile phase of acetonitrile, water, ammonium acetate, acetic acid and 0.02 M triethylamine. The limit of quantitation of amphotericin B was 1 ng ml−1. Precision and accuracy was <5%.

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Authors and Affiliations

  1. Servicio de Farmacología Clínica, Clínica Universitaria de Navarra, 31008, Pamplona, Spain

    M. A. Campanero & J. R. Azanza

  2. Centro Galénico, Universidad de Navarra, Ap. 177, 31080, Pamplona, Spain

    M. S. Espuelas & J. M. Irache

Authors
  1. M. A. Campanero
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  2. M. S. Espuelas
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  3. J. R. Azanza
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  4. J. M. Irache
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Campanero, M.A., Espuelas, M.S., Azanza, J.R. et al. Rapid determination of intramacrophagic amphotericin B by direct injection HPLC. Chromatographia 52, 827–830 (2000). https://doi.org/10.1007/BF02491013

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  • DOI: https://doi.org/10.1007/BF02491013

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