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Two-dimensional thin-layer chromatography of plant ecdysteroids

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Summary

Two-dimesional thin-layer chromatography has been used to screen plant samples for ecdysteroids. Satisfactory separations were obtained even in the presence of substantial impurities and for mixtures containing many ecdysteroids. Normal-phase silica and octadecyl silica plates were used to differntiate apolar and polar ecdysteroids, respectively. The major advantages of thin-layer chromatography are multiple detection, which enables specific detection of the ecdysteroids. Use of a small amount of water in the mobile phase in the fist dimension, the a water-free mobile phase in the second dimension, can afford favorable separations.

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Authors and Affiliations

  1. Department of Pharmacognosy, University of Szeged, 6720, Szeged, Eötyös u 6, Hungary

    M. Báthori

  2. Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, UK

    G. Blunden

  3. Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyváradtér 4, P.O. Box 370, 1445, Budapest, Hungary

    H. Kalász

Authors
  1. M. Báthori
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  2. G. Blunden
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  3. H. Kalász
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Báthori, M., Blunden, G. & Kalász, H. Two-dimensional thin-layer chromatography of plant ecdysteroids. Chromatographia 52, 815–817 (2000). https://doi.org/10.1007/BF02491010

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  • DOI: https://doi.org/10.1007/BF02491010

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