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Identification of FC/TA-891 and its metabolite, FCE22101 by high performance liquid chromatography — Atmospheric pressure chemical lonization mass spectrometry using bromoform

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Summary

A new identification method was developed for a heat-unstable antibiotic, FC/TA-891, which belongs to the penem type antibiotic, and its active metabolite (FCE22101). High-performance liquid chromatography (HPLC)—atmospheric pressure chemical ionization mass spectrometry (APCI-MS) was employed for their identification. As an ionization acceleration solvent, bromoform, which was added to the mobile phase in HPLC, was found to give clear and stable adductions, [M+Br]− for FC/TA-891 and FCE22101, compared to chloroform. These adduct ions can be clearly distinguished from other ions due to their isotopical ratio (1∶1), leading to an easy identification of both compounds.

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Authors and Affiliations

  1. Analytical Research Laboratory, Tanabe Seiyaku Co., Ltd. 16-89, Kashima 3-chome, Yodogawa-ku, 532-8505, Osaka, Japan

    S. Horimoto, T. Mayumi, K. Aoe & N. Nishimura

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  1. S. Horimoto
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  2. T. Mayumi
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  3. K. Aoe
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  4. N. Nishimura
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Horimoto, S., Mayumi, T., Aoe, K. et al. Identification of FC/TA-891 and its metabolite, FCE22101 by high performance liquid chromatography — Atmospheric pressure chemical lonization mass spectrometry using bromoform. Chromatographia 52, 741–744 (2000). https://doi.org/10.1007/BF02490999

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  • DOI: https://doi.org/10.1007/BF02490999

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