Chromatographia

, Volume 52, Issue 11–12, pp 735–740 | Cite as

A high-performance liquid chromatographic method for screening angiotensin II receptor antagonists in human urine

  • L. González
  • R. M. Alonso
  • R. M. Jiménez
Originals Column Liquid Chromatography

Summary

A high-performance liquid chromatographic method with photometric detection has been developed for the determination of five angiotensin II receptor antagonists (ARA II): Losartan, Irbesartan, Valsartan, and Candesartan cilexetil and its metabolite Candesartan M1. Reversedphase chromatography was performed at room temperature on a μBondapak C18 column; compounds were separated by gradient elution with mixtures of acetonitrile and 0.1 M acetate buffer, pH 4, at different flow rates. Detection was at 254 nm. Urine samples were purified by solid-phase extraction on C8 cartridges, a procedure enabling recoveries > 85% for all the drugs except Candesartan cilexetil (71%). The analysis time was less than 50 min, including extraction (chromatographic analysis lasted 25 min). Intra- and inter-day coefficients of variation for all the compounds were below 8% and the method was highly accurate (relative error,RE, usually ca 3%). The accuracy, precision, and sensitivity (limit of quantitation 0.4 μg mL−1) of the method were sufficient for application to the screening of these ARA II in spiked urine samples.

Key words

Column liquid chromatography Angiotensin II receptor antagonists Screening Urine 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • L. González
    • 1
  • R. M. Alonso
    • 1
  • R. M. Jiménez
    • 1
  1. 1.Departamento de Química Analítica, Facultad de CienciasUniversidad dal país VascoBilbaoSpain

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