Summary
A capillary isoelectric focusing (CIEF) method under denaturing condition has been developed for separation of α-keratins, highly cross-linked biomacromolecules, in horns of rhinoceros and buffalo. The α-keratins were denatured in 8 M urea and separated in the presence of ampholytes with applicable pH ranges of 3–7 or 3–10. In the preliminary results, it was demonstrated that the α-keratins in the horns of buffalo or rhinoceros might have their own unique isoelectric focusing profiles. The analyses of more samples from different rhinoceros and buffalo are presently underway, with the final goal to establish a fast and convenient method for identification of the source (buffalo or rhinoceros) of an unknown powdered sample.
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Huang, T.L., Yu, P. Analysis of α-keratins in the horns of rhinoceros and buffalo by non-native capillary isoelectric focusing. Chromatographia 46, 437–439 (1997). https://doi.org/10.1007/BF02490883
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DOI: https://doi.org/10.1007/BF02490883