, Volume 46, Issue 7–8, pp 399–403 | Cite as

Rapid isocratic HPLC analysis of beta-carotene in red peppers (Capsicum annuum L.) and food preparations

  • M. Weissenberg
  • A. Levy
  • I. Schaeffler
  • E. Menagem
  • M. Barzilai


A simple, rapid high-performance liquid chromatography method has been devised in order to separate and quantify β-carotene present in red pepper (Capsicum annuum L.) fruits and food preparations. A reversed-phase isocratic non-aqueous system enables the separation of β-carotene within a few minutes, with detection at 450 nm. The selection of extraction solvents, mild saponification conditions, and chromatographic features, is evaluated and discussed. The method is proposed for rapid screening of large plant populations, plant selection, as well as for food products, and also for nutrition and quality control studies.

Key Words

Column liquid chromatography β-Carotene Capsanthin and Capsorubin Red peppers 


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  1. [1]
    J. C. Bauerfeind, G. B. Brubacher, H. M. Kläui, W. L. Marusich, in “Carotenoids”, O. Isler, Ed., Birkhäuser Verlag, Basel, 1971; p. 743.Google Scholar
  2. [2]
    JECFA (Joint FAO/WHO Expert Committee on Food Additives) Compendium of Food Additive Specifications, vol. 1, FAO, Rome, 1992; pp. 383 and 385.Google Scholar
  3. [3]
    J. A. Olson, J. Nutr.119, 105 (1989).Google Scholar
  4. [4]
    G. W. Burton, J. Nutr.119, 109 (1989).Google Scholar
  5. [5]
    R. G. Ziegler, J. Nutr.119, 116 (1989).Google Scholar
  6. [6]
    H. Gerster, Int. J. Vit. Nutr. Res.63, 93 (1993).Google Scholar
  7. [7]
    A. Bendich, J. Nutr.119, 112 (1989).Google Scholar
  8. [8]
    N. I. Krinsky, J. Nutr.119, 123 (1989).Google Scholar
  9. [9]
    J. Blumberg, G. Block, Nutr. Rev.52, 242 (1994).CrossRefGoogle Scholar
  10. [10]
    M. Nicol, M. Maudet, N. Savoure, Med. Nutr.30, 212 (1994).Google Scholar
  11. [11]
    K. Kleiner, New Scientist149, 4 (1996).Google Scholar
  12. [12]
    R. J. Bushway, J. Liq. Chromatogr.8, 1527 (1985).Google Scholar
  13. [13]
    M. Ruddat, O. H. Will III, Methods Enzymol.111, 189 (1985).Google Scholar
  14. [14]
    A. P. De Leenheer, H. J. Nelis, W. E. Lambert, R. M. Bauwens, J. Chromatogr.429, 3 (1988).Google Scholar
  15. [15]
    F. Khachik, G. R. Beecher, M. B. Goli, W. R. Lusby, Methods Enzymol.213, 347 (1992).Google Scholar
  16. [16]
    N. E. Craft, S. A. Wise, J. H. Soares, Jr., J. Chromatogr.589, 171 (1992).CrossRefGoogle Scholar
  17. [17]
    K. S. Epler, L. C. Sander, R. G. Ziegler, S. A. Wise, N. E. Craft, J. Chromatogr.595, 89 (1992).CrossRefGoogle Scholar
  18. [18]
    E. Lesellier, A. Tchapla, C. Marty, A. Lebert, J. Chromatogr.633, 9 (1993).CrossRefGoogle Scholar
  19. [19]
    M. Weissenberg, I. Schaeffler, E. Menagem, M. Barzilai, A. Levy, J. Chromatogr. A757, 89 (1997).CrossRefGoogle Scholar
  20. [20]
    G. Britton, Methods Enzymol.111, 113 (1985).CrossRefGoogle Scholar
  21. [21]
    C. A. Pesek, J. J. Warthesen, P. S. Taoukis, J. Agric. Food Chem.38, 41 (1990).CrossRefGoogle Scholar
  22. [22]
    M. Kimura, D. B. Rodriguez-Amaya, H. T. Goodoy, Food Chem.35, 187 (1990).CrossRefGoogle Scholar
  23. [23]
    H. Nyambaka, J. Ryley, Food Chem.55, 63 (1996).CrossRefGoogle Scholar
  24. [24]
    N. E. Craft, J. H. Soares, Jr., J. Agric. Food Chem.40, 431 (1992).CrossRefGoogle Scholar
  25. [25]
    I. Stewart, T. A. Wheaton, Phytochemistry12, 2947 (1973).CrossRefGoogle Scholar
  26. [26]
    K. J. Scott, Food Chem.45, 357 (1992).CrossRefGoogle Scholar
  27. [27]
    K. J. Scott, D. J. Hart, Food Chem.47, 403 (1993).CrossRefGoogle Scholar
  28. [28]
    D. J. Hart, K. J. Scott, Food Chem.54, 101 (1995).CrossRefGoogle Scholar
  29. [29]
    N. E. Craft, L. C. Sander, H. F. Pierson, J. Micronutr. Anal.8, 209 (1991).Google Scholar
  30. [30]
    F. Khachik, G. R. Beecher, J. T. Vanderslice, G. Furrow, Anal. Chem.60, 807 (1988).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1997

Authors and Affiliations

  • M. Weissenberg
    • 1
  • A. Levy
    • 1
  • I. Schaeffler
    • 1
  • E. Menagem
    • 1
  • M. Barzilai
    • 1
  1. 1.Laboratory of Natural Products Chemistry, Department of Plant Genetics, Agricultural Research OrganizationThe Volcani CenterBet DaganIsrael

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