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Assay ofpara-aminobenzoic acid formed by hydrolysis of procaine in CP1B solution

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  • Thin-Layer Chromatography
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Summary

CP1 is a cardioplegia solution containing procaine, a substance that is unstable at pH>7. For this reason the product it is supplied as two solutions that are mixed immediately before use.

para-Aminobenzoic acid (PABA) was separated by horizontal high-performance thin-layer chromatography (HPTLC) on silica gel 60 nanoplates with fluorescence indicator. This method is specific, repeatable, and reproducible.

The autoclave sterilization conditions currently used are the least aggressive towards procaine. Under normal storage conditions, the concentration of procaine in CP1B complies with standards (47.5–52.5 mmol L−1) for a period of four years. The shelf-life of 2 years is thus satisfactory.

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Authors and Affiliations

  1. Service de Pharmacie, Hôpital Broussais, 96, Rue Didot, 75014, Paris, France

    Ph. Galais & A. Chevallier

  2. Laboratoire d’analyses physico-chimiques, Pharmacie Centrale des Hôpitaux (PCH) Assistance Publique-Hôpitaux de Paris, 7 Rue du Fer à Moulin, 75005, Paris, France

    C. Dauphin & D. Pradeau

Authors
  1. Ph. Galais
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  2. C. Dauphin
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  3. D. Pradeau
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  4. A. Chevallier
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Galais, P., Dauphin, C., Pradeau, D. et al. Assay ofpara-aminobenzoic acid formed by hydrolysis of procaine in CP1B solution. Chromatographia 52, 115–119 (2000). https://doi.org/10.1007/BF02490804

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  • DOI: https://doi.org/10.1007/BF02490804

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