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Chromatographia

, Volume 52, Issue 1–2, pp 103–108 | Cite as

Determination of the oil content of seeds by focused microwave-assisted soxhlet extraction

  • L. E. García-Ayuso
  • J. Velasco
  • M. C. Dobarganes
  • M. D. Luque de Castro
Originals Supercritical Fluid Extraction

Sumamry

Soybean, rape and sunflower lipids have been extracted with a focused microwave-assisted Saxhlet extractor. The main factors affecting extraction efficiency, namely microwave irradiation power, number of cycles and microwave irradiation time were optimised by means of a power, number of cycles and microwave irradiation time were optimised by means of a central composite design based on a two-level-three-factor factorial design. A study of the influence of particle size on the extraction procedure was also performed. The results obtained were compared with those obtained by use of an ISO method using a conventional Soxhlet extractor. Quantitative results for lipid content based on gravimetric determinations and qualitative results based on analysis of fatty acid methyl esters and polymeric compounds were similar to those obtained by Soxhlet extraction with hexane. Substantial reduction of sample manipulation. analysis time and solvent wastage is achieved by use of the proposed method.

Key Words

Microwave-assisted Soxhlet extraction Lipid extraction Oleaginous seeds Factorial design 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • L. E. García-Ayuso
    • 1
  • J. Velasco
    • 2
  • M. C. Dobarganes
    • 2
  • M. D. Luque de Castro
  1. 1.Analytical Chemistry Division, Faculty of SciencesUniversity of CórdobaSpain
  2. 2.Instituto de la Grasa y derivadosConsejo Superior de Investigaciones Científicas (CSIC)SevillaSpain

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