Summary
A new method of the determination of the diffusion coefficients,D, called “the peak-height method” has been developed. When the zone broadening of a species is solely caused by axial molecular diffusion, its diffusion coefficient can be calculated.
The diffusion coefficients of L-cysteine and glutathione in a neutral phosphate buffer were measured by this method. Comparison between the experimental diffusion coefficient and the one calculated by the Wilke-Chang equation shows that the method gives good results.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Jorgenson, J.W.; Lukacs, K.D.Science 1983, (Washington, D.C.)222, 266.
Ewing, A.G.; Wallingford, R.A.; Olefirowica, T.M.Anal. Chem. 1989,61, 292A.
Gordon M.J.; Huang, X.; Pentoney, S.J. Jr.; Zare, R.N.;Science 1988, (Washington, D.C.),242, 224.
Yik, Y.F.; Li, S.F.Y.Trends in Anal. Chem. 1992,11(9), 325.
Walbroehl, Y.; Jorgenson, J.W.J. Microcol. Sep. 1989,1(1), 41.
Huang, X.H.; Coleman, W.F.; Zare, R.N.J. Chromatogr. 1989,480, 95.
Jones, H.K.; Nguyen, N.T.; Smith, R.D.J. Chromatogr. 1990,504, 1.
Crank, J.The Mathematics of Diffusion 1975, 2nd ed., Clarendon Press, Oxford, UK.
Jin, W.; Chen, H.J. Chromatogr. A 1997,765, 307.
Bard, A.J.; Faulkner, L.R.Electrochemical Methods 1980, 668. John Wiley & Sons, Inc., New York, USA.
Jin, W.; Chen, H.J. Electroanal. Chem. 1997,425, 155.
Kolthoff, I.M.; Barnum, C.J. Amer. Chem. Soc. 1940,62, 3061.
Stankorich, M.T.; Bard, A.J.J. Electroanal. Chem. 1977,75, 487.
Stricks, W.; Kolthoff, I.M.J. Am. Chem. Soc. 1952,74, 4646.
Knox, J.H.,Chromatographia 1988,26, 329.
Mikkers, F.E.P.; Everaerts, F.M.; Verheggen, Th.P.E.M.J. Chromatogr. 1979,169, 11.
Jones, H.K.; Nguyen, N.T.; Smith, R.D.J. Chromatogr. 1990,504, 1.
O’Shea, T.J.; Lunte, S.M.Anal. Chem. 1993,65, 247.
Gussler, E.L.Diffusion Mass transfer in fluid systems 1984, 121. Cambridge University Press, Cambridge.
Barrett, G.C.Chemistry and Biochemistry of the Amino Acids 1985, 596. Chapman and Hall Ltd., London.
Knox, J.H.; Macormack, K.A.Chromatographia 1994,38, 207.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jin, W., Chen, H. A new method of determination of diffusion coefficients using capillary zone electrophoresis (peak-height method). Chromatographia 52, 17–21 (2000). https://doi.org/10.1007/BF02490786
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02490786