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Direct determination of sialic acids in serum by capillary electrophoresis with UV detection

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  • Capillary Electrophoresis
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Summary

A novel method for the determination of N-acetylneuraminic acid (NANA) and N-glycolylneuraminic acid (NGNA) has been developed using high-performance capillary electrophoresis with UV detection at 195 nm, without pre or post-column derivatisation. The acids were separated in a 50-cm, fused-silica capillary (50μ i.d, 45.5-cm effective length) with Na2B4O7−Na2HPO4 buffer. The detection limit for NANA is a concentration of 9.6×10−6 M or, in terms of mass:3.879×10−14 mol (39 fmol). This method is applicable to determination of NANA in normal human serum. The results were also compared with those of the colorimetrie method.

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Authors and Affiliations

  1. Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, 457 Zhongshan Road, 116023, Dalian, P.R. China

    X. L. Dong & B. C. Lin

  2. Dalian Third Municipal Hospital, 40 Qianshan Road, 116033, Dalian, P.R. China

    X. L. Dong

  3. Dalian Obstetrics and Gynecology Hospital, 162 Zhongshan Road, 116012, Dalian, P.R. China

    Y. Xu

Authors
  1. X. L. Dong
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  2. Y. Xu
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  3. B. C. Lin
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Dong, X.L., Xu, Y. & Lin, B.C. Direct determination of sialic acids in serum by capillary electrophoresis with UV detection. Chromatographia 52, 14–16 (2000). https://doi.org/10.1007/BF02490785

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  • DOI: https://doi.org/10.1007/BF02490785

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