Summary
The potentiality of a porous graphitized carbon stationary phase was assessed for ceramide analysis in nonaqueous reversed phase chromatography with an evaporative light scattering detection. The influence of the nature of the mobile phase was first performed under isoeluotropic isocratic conditions. Eight mobile phase compositions in the presence or absence of triethylamine/formic acid were tested with a set of structurally relevant ceramides. Values obtained under these conditions for the peak heights, retention factors, plate numbers, asymmetry factors, relative retentions and Time-Corrected Calibrated Normalised Resolution Product were analysed by Principal Component Analysis. Principal Component Analysis was effective in attributing relationships between the chromatographic behaviour and analytical conditions. The relationship between the increase of the detector response and the presence of triethylamine/formic acid has been confirmed, and the main change of selectivity has been attributed to interactions provided by the weak solvent of the mobile phase. These conclusions have allowed the development of gradient conditions for the analysis of complex ceramide mixtures on porous graphitized carbon column.
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Gaudin, K., Chaminade, P., Ferrier, D. et al. Use of principal component analysis for investigation of factors affecting retention behaviour of ceramides on porous graphitized carbon column. Chromatographia 50, 470–478 (1999). https://doi.org/10.1007/BF02490744
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DOI: https://doi.org/10.1007/BF02490744