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Chromatographia

, Volume 50, Issue 7–8, pp 457–460 | Cite as

A high-performance liquid-chromatographic method for the determination of ochratoxin a in human plasma

  • A. M. Jimenez
  • A. López de Cerain
  • E. Gonzalez-Peñas
  • J. Bello
Originals

Summary

A high-performance liquid-chromatographic method is described for the quantitative determination of the mycotoxin ochratoxin A (OTA) in human plasma. The assay involves extraction with chloroform and sodium bicarbonate then HPLC with fluorescence detection. The method was validated in terms of selectivity, recovery, linearity, precision (within-day and between-day variability), accuracy, detection and quantification limits, and the stability of OTA in plasma and treated samples. The limit of detection was 0.4 ng mL−1 of OTA in methanol, corresponding to 0.52 ng ml−1 OTA in plasma. This assay was successfully applied for the determination of OTA levels in human plasma.

Key Words

Column liquid chromatography Ochratoxin A Mycotoxins Validation 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1999

Authors and Affiliations

  • A. M. Jimenez
    • 1
  • A. López de Cerain
    • 1
  • E. Gonzalez-Peñas
    • 2
  • J. Bello
    • 1
  1. 1.Department of Food Science and Toxicology, Faculty of PharmacyUniversity of NavarraPamplonaSpain
  2. 2.Department of Organic and Pharmaceutical Chemistry, Faculty of PharmacyUniversity of NavarraPamplonaSpain

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