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Chromatographia

, Volume 50, Issue 7–8, pp 433–438 | Cite as

Analysis of flavonols ofSedum telephium L. leaves by capillary electrophoresis and HPLC-mass spectrometry

  • S. Sturm
  • N. Mulinacci
  • F. F. Vincieri
  • H. Stuppner
Originals

Summary

Flavonol glycosides ofSedum telephium L. were analysed by MEKC. Baseline separation was achieved within 14 min using a fused silica capillary and a borate/phosphate buffer solution (35 mM, pH 5.8) containing 35 mM SDS and 4% MeOH. The applied voltage was 25 kV and the thermostating temperature was kept constant at 40°C. Injection was performed via the pressure mode for 3 s, the detection wavelength was 205 nm. The optimized MEKC method was used for the quantitative determination of flavonol glycosides in extracts ofS. telephium leaves.

Analysis was also performed by HPLC-MS using an electrospray ionization (ESI) interface. The good agreement between the quantitative CE results and those obtained by LC clearly demonstrated the applicability of the methods presented.

Key Words

Column liquid chromatography Micellar electrokinetic chromatography Mass spectrometry Electrospray ionization Flavonol glycosides 

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References

  1. [1]
    H. Rzadkowska-Bodalska in “Hagers Handbuch der Pharmazeutischen Praxis”, 5th edn., Vol. 5, R. Hänsel, K. Keller, H. Rimpler, G. Schneider, Eds., Springer-Verlag, Berlin, Heidelberg, New York., 1994, p. 650.Google Scholar
  2. [2]
    A. Sendl, N. Mulinacci, F.F. Vincieri, H. Wagner, Phytochemistry34, 1357 (1993).CrossRefGoogle Scholar
  3. [3]
    N. Mulinacci, F.F. Vincieri, A. Baldi, M. Bambagiotti-Alberti, A. Sendl, H. Wagner, Phytochemistry38, 531 (1995).CrossRefGoogle Scholar
  4. [4]
    N. Mulinacci, F.F. Vincieri, A. Baldi, A. Romani, D. Favretto, P. Traldi, Rapid Communications in Mass Spectrometry9, 963 (1995).CrossRefGoogle Scholar
  5. [5]
    K. V. Casteele, H. Geiger, Ch. Van Sumere, J. Chromatog.,240, 81 (1982).CrossRefGoogle Scholar
  6. [6]
    D. J. Daigle, E.J. Conkerton, J. Chromat.240, 202 (1992).Google Scholar
  7. [7]
    T.K. McGhie, J. Chromat.634, 107 (1993).CrossRefGoogle Scholar
  8. [8]
    Ph. Morin, F. Villard, M. Dreux, J. Chromat.628, 161 (1993).CrossRefGoogle Scholar
  9. [9]
    U. Seitz, P. G. Oefner, M. Popp, J. Chromat.559, 499 (1991).CrossRefGoogle Scholar
  10. [10]
    P.G. Pietta, P.L. Mauri, L. Zini, C. Gardana, J. Chromat. A680, 175 (1994).CrossRefGoogle Scholar
  11. [11]
    J. Cai, J. Henion, J. Chromat. A703, 667 (1995).CrossRefGoogle Scholar
  12. [12]
    C. O. Lehmann in “Urania Pflanzenreich, Blütenpflanzen 1, Uraniaverlag Leipzig, 1985, p. 163.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1999

Authors and Affiliations

  • S. Sturm
    • 1
  • N. Mulinacci
    • 2
  • F. F. Vincieri
    • 2
  • H. Stuppner
    • 1
  1. 1.Institute of PharmacognosyUniversity of InnsbruckAustria
  2. 2.Department of Pharmaceutical ScienceUniversity of FlorenceItaly

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