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Rapid and specific determination of free cortisol in guinea pig urine and faeces by thin-layer chromatography-competitive protein-binding assay

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Summary

The present work describes a specific, rapid and sensitive determination of free cortisol in the urine and faeces of guinea pigs. This method offers some advantages over previous methods including the use of dexamethasone as a marker for cortisol localization on TLC sheets, the use of buffer instead of organic solvents to recover cortisol from silica gel 60 and the rapidity of the competitive protein-binding assay (1–2 h).

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Authors and Affiliations

  1. Department of Animal Physiology, NW I, University of Bayreuth, 95440, Bayreuth, Germany

    M. Fenske

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  1. M. Fenske
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Fenske, M. Rapid and specific determination of free cortisol in guinea pig urine and faeces by thin-layer chromatography-competitive protein-binding assay. Chromatographia 50, 428–432 (1999). https://doi.org/10.1007/BF02490737

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  • DOI: https://doi.org/10.1007/BF02490737

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