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Enhanced sensitivity by laser-induced fluorescence for the determination of calcitriol and other vitamin D3 metabolites in plasma

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Summary

A sensitive method for the determination of hydroxymetabolites of vitamin D3, parficularly calcitriol (1,25-(OH)2-D3), in human plasma is reported. The method is based on the use of laser-induced fluorescence detection as an alternative to conventional fluorimetry in an integrated cleanup/preconcentration, HPLC separation and post-column derivatization system. The derivatization step is based on a dehydration reaction which takes place in secosteroid structures at high temperature in a strong-acid medium. A LOD of 0.01 pg mL−1 (SNR=3) was obtained for each analyte with a linear dynamic range over 4 order of magnitude with excellent regression coefficients (≥0.9922) in all cases. The precision was studied at two concentration levels and the RSDs values (for n=5) were acceptable (between 2.6 and 4.7%). The method was also checked by applying it to human plasma spiked with the target analytes and excellent recoveries were obtained. This is the first time that these species have been determined at the sub-pg mL−1 level.

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References

  1. A. Brown, A. Dusso, E. Slatopolsky, Vitamin D. In D. W. Seldin, and G. Giebisch, Eds., The kidney: physiology and physiopathology, 2nd ed. New York; Raven Press, 1992.

    Google Scholar 

  2. N. H. Bell, P. H. Stern, E. Panther, T. K. Sinha, H. F. DeLuca, J. Clin. Invest.64, 218 (1979).

    Article  CAS  Google Scholar 

  3. E. Abe, C. Miyaura, H. Sakagami, M. Takeda, K Konno, T. Yamazaki, S. Yoshiki, T. Suda, Proc. Acad. Sci.78, 4990 (1981).

    Article  CAS  Google Scholar 

  4. T Suda, T. Shinki, N. Takahashi, Annu. Rev. Nutr.10, 195 (1990).

    Article  CAS  Google Scholar 

  5. Y. J. Zhou, A. W. Norman, M. Lübbert, E. D. Collimns, M. R. Uskokovic, H. P. Koeffler, Blood,74, 82 (1989),

    CAS  Google Scholar 

  6. G. Jones, D. H. J. Trafford, H. L. J. Makin, B. W Hollis, Vitamin D: Cholecalciferol, Ergocalciferol and hydroxylated metabolites. In A. P. Delleheer, w. E. Lambert and H. J. Nelis Eds., Modern Chromatographic Analysis of Vitamins; Chromatographic Science Series. Marcel Dekker. Vol.60, 1992

  7. K Shimada, N. Kobayashi, Trends Anal, Chem.10 (3), 103 (1991)

    Article  CAS  Google Scholar 

  8. H. Koyama, J. M. Prahl, A. Uhland, M. Nanjo, M. Inaba, Y. Nishizawa, Y. Morii, Y. Nishii, H. F. DeLuca, Anal. Biochem,205 (2), 213 (1992)

    Article  CAS  Google Scholar 

  9. M. L. Traba, M. Babe, C. De-la-Piedra, A. Marin, Clin. Chim. Acta.29 (10), 1806 (1983)

    CAS  Google Scholar 

  10. F. H. Johannsen, Landwirtsch. Forsch.40 (1), 32 (1987)

    CAS  Google Scholar 

  11. M. A. Haughton, R. S. Mason, Clin. Chem.38 (9), 179 (1992).

    Google Scholar 

  12. E. Stary, A. M. C. Cruz, C. A. Donomai, J. L. Mondardinl. J. High Resolut. Chromatogr,12 (6), 421 (1989).

    Article  CAS  Google Scholar 

  13. E. C. Bligh, W. J. A. Dyer, Can. J. Biochem. Physiol.37, 911, (1959)

    CAS  Google Scholar 

  14. G. Jones, Clin. Chem.24, (2), 287 (1978).

    CAS  Google Scholar 

  15. M. Shimizu, Y. Gao, T. Aso, K. Nakatsu, S. Yamada, Anal. Biochem.204 (2), 258 (1992).

    Article  CAS  Google Scholar 

  16. R. D. Coldwell, D. J. H. Trafford, H. L. J. Makin, Clin Chem.31 (11), 1919 (1985).

    CAS  Google Scholar 

  17. R. D. Coldwell, D. J. H. Trafford, M. J. Varley, D. N. Kirk, H. L. J. Makin, Clin. Chim. Acta180 (2), 157 (1989).

    Article  CAS  Google Scholar 

  18. F. Ortiz-Boyer, J. M. Fernández-Romero, M. D. Luque de Castro J. M. Quesada, J. Chromatogr. B.693, 43 (1997).

    Google Scholar 

  19. R. D. Coldwell, D. J. H. Trafford, H. L. J. Makin, M. J. Varley, D. N. Kirk, J. Chromatogr. Biomed. Appl.39, 2891 (1985).

    Google Scholar 

  20. E. B. Mawer, J. T. Hann, J. Chromatogr. Biomed. Appl.59, 305 (1987).

    Google Scholar 

  21. R. L. G. Norris, M. J. Thomas, P. W. Craswell, Chromatogr. Biomed. Appl.54 (1), 53 (1986).

    Article  Google Scholar 

  22. S. Shimada, K. Mitamura, N. Kitama, Biomed. Chromatogr.9 (5), 229 (1995).

    Article  CAS  Google Scholar 

  23. A. Benmoussa, J. L. Lacout, P. R. Loiseau, M. Mikou, Chromatographia42 (3–4), 177 (1996).

    Article  CAS  Google Scholar 

  24. A. Benmoussa, G. Dlaurent, J. L. Lacout, P. R. Loiseau, M. Mikou, J. Chromatogr.731 (1–2), 153 (1996).

    Article  CAS  Google Scholar 

  25. J. M. Halket, I. Ganschow, B. P. Lisboa, J. Chromatogr.192 (2), 434 (1980).

    CAS  Google Scholar 

  26. S. Komuro, I. Nakatsuka, A. Yoshizake, K. Iba, Biol. Mass. Spectrom.23 (1), 33 (1994).

    Article  CAS  Google Scholar 

  27. R. D. Coldwell, D. J. L. Trafford, H. L. J. Makin, J. Mass. Spec.30, 348 (1995).

    Article  CAS  Google Scholar 

  28. P. P. Nair, C. Bucana, D. DeLeón, D. A. Turner, Anal. Chem.37, 631 (1965).

    Article  CAS  Google Scholar 

  29. I. Bjorkhem, I. Holmberg, T. Kristiansen, J. I. Pedersen, Clin. Chem.25 (4), 584 (1979).

    CAS  Google Scholar 

  30. P. M. K. Poon, I. T. Mak, C. P. Pang, Clin. Biochem.26 (6), 461 (1993).

    Article  CAS  Google Scholar 

  31. K. Shimada, K. Mitamura, M. Mukoujamaj, T. Okura, K. Sagaya, J. Chromatogr. Sci.32 (2), 82 (1995).

    Google Scholar 

  32. D. A. Seamark, D. J. H. Trafford, P. G. Hiscocks, H. L. J. Makin, J. Chromatogr.197 (2), 271 (1980).

    Article  CAS  Google Scholar 

  33. T. Iwata, M. Yamaguchi, H. Hanazono, Y. Imeto M. Nakamura, Y. Ohkura, Anal. Sci.6 (3), 361 (1990).

    CAS  Google Scholar 

  34. K. Shimada, I. Nakatani, K. Saito, K. Mitamura, Biol. Pharm. Bull.19 (4), 491 (1996).

    CAS  Google Scholar 

  35. P. H. Jordan, G. Read, T. Hargreaves, Analyst.116 (12), 1347 (1991).

    Article  CAS  Google Scholar 

  36. B. Yeung, P. Vouros, G. S. Reddy, J. Chromatogr.645 (1), 115 (1993).

    Article  CAS  Google Scholar 

  37. R. J. Vreeken, M. Honing, B. L. M. Van-Baar, R. T. Ghijsen, G. J. DeJong, U. A. T. Brinkman, Biol. Mass-Spectrom.22 (11), 621 (1993).

    Article  CAS  Google Scholar 

  38. D. J. H. Trafford, D. A. Seamark, H. Turnbull, H. L. J. Makin, J. Chromatogr. Biomed. Appl.15, 351 (1981).

    Google Scholar 

  39. M. Shimizu, Y. Iwasaki, H. Ishida, S. Yamada, J. Chromatogr.672, 63 (1995).

    CAS  Google Scholar 

  40. T. Iwata, M. Yamaguchi, H. Hanazono, Y. Imazato, M. Nakamura, Y. Ohkura, Anal. Sci.6 (3), 361 (1990).

    CAS  Google Scholar 

  41. M. Shimizu, S. Kamachi, Y. Nishii, S. Yamada, Anal. Biochem.194, 77 (1991).

    Article  CAS  Google Scholar 

  42. A. Sánchez-Pérez, M. J. Gallego-Matilla, J. S. Hernández-Méndez, Anal. Lett.26 (4), 721 (1993).

    Google Scholar 

  43. S. A. Solpe, S. M. Lunte, T. Kuwana, Anal. Sci.5, 23 (1989).

    Google Scholar 

  44. R. J. Van de Nesse, G. P. Hoornwegm, C. Gooijer, U. A. T. Brinkman, N. H. Velthorst, Anal. Chim. Acta.227, 173 (1989).

    Article  Google Scholar 

  45. S. V. Rahavendran, H. T. Karnes, Anal. Chem.69 (15), 3022 (1997).

    Article  CAS  Google Scholar 

  46. D. L. Stokes, M. J. Sepaniak, T. Vo-Dinh, Biomed. Chromatogr11 (4), 187 (1997).

    Article  CAS  Google Scholar 

  47. E. Gonzalez, J. J. Laserna, Quim. Anal.16, 3–15 (1997).

    CAS  Google Scholar 

  48. H. Li, H. Shang, C. Liu, Shenyang-Yaoxueyuan-Xuebao8 (4), 248 (1991).

    CAS  Google Scholar 

  49. F. Ortiz-Boyer, J. M. Fernández-Romero, M. D. Luque de Castro, J. M. Quesada, Clin. Chim. Acta.274, 139 (1998).

    Article  CAS  Google Scholar 

  50. F. Ortiz-Boyer, J. M. Fernández-Romero, M. D. Luque de Castro, J. M. Quesada, Talanta. (in press, 1998).

  51. F. Ortiz-Boyer, J. M. Fernández-Romero, M. D. Luque de Castro, J. M. Quesada, J. Liq. Chrom. & Rel. Technol.21 (4), 503 (1998).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Analytical Chemistry Division, Faculty of Sciences, University of Córdoba, 14004, Spain

    F. Ortiz Boyer, J. M. Fernández Romero & M. D. Luque de Castro

  2. Mineral Metabolism Unit, Hospital Reina Sofia, University of Córdoba, 14004, Córdoba, Spain

    J. M. Quesada

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  1. F. Ortiz Boyer
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  2. J. M. Fernández Romero
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  3. M. D. Luque de Castro
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  4. J. M. Quesada
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Ortiz Boyer, F., Fernández Romero, J.M., Luque de Castro, M.D. et al. Enhanced sensitivity by laser-induced fluorescence for the determination of calcitriol and other vitamin D3 metabolites in plasma. Chromatographia 50, 399–406 (1999). https://doi.org/10.1007/BF02490733

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  • DOI: https://doi.org/10.1007/BF02490733

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