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The use of capillary electrophoresis with entangled polymer matrix to analyse plasmid DNA and a cationic lipid/cholesterol liposome: DNA complex

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Summary

A liposome based gene therapeutic product is being developed for the treatment of cystic fibrosis. The product comprises a complex of plasmid DNA and a cationic lipid/cholesterol liposome. Using CE with an entangled polymer matrix, routine separations of linear, supercoiled and open-circle conformers of DNA in plasmid DNA and the liposome complex have been performed. The CE method has been used to support novel quality control and process validation for the manufacture of plasmid DNA and to monitor the degradation of DNA in the liposome complex. Significant features of the method are simple sample preparation and the use of direct UV detection, avoiding the use of potentially mutagenic reagents.

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Authors and Affiliations

  1. Glaxo Wellcome Research and Development, Park Road, SG12 ODP, Ware, Herts., UK

    M. J. Orchard, I. P. Collin & M. Bushell

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  1. M. J. Orchard
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  2. I. P. Collin
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  3. M. Bushell
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Orchard, M.J., Collin, I.P. & Bushell, M. The use of capillary electrophoresis with entangled polymer matrix to analyse plasmid DNA and a cationic lipid/cholesterol liposome: DNA complex. Chromatographia 50, 181–187 (1999). https://doi.org/10.1007/BF02490649

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  • DOI: https://doi.org/10.1007/BF02490649

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