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Chromatographia

, Volume 51, Issue 3–4, pp 163–166 | Cite as

Comparison of different methods for the determination of several quinolonic and cinolonic antibiotics in trout muscle tissue by HPLC with fluorescence detection

  • I. Durán Merás
  • T. Galeano Díaz
  • F. Salinas López
  • M. I. Rodríguez Cacéres
Originals

Summary

Quinolonic and cinolonic derivatives are mainly used as antibacterials in fish-farms. In this paper we describe a careful revision of the treatment procedures of samples and a prodedure for the determination of residues of these compounds. Because of the complexity and duration of these procedures, several studies have been carried out and these have lead to a simpler and shorter method. Three approaches have been examined: lyophilization followed by extraction with chloroform, solid-liquid extraction with chloroform and solid-liquid extraction with sodium hydroxide solution, followed by liquid-liquid partition in chloroform. Some previous studies into the partition equilibrium are also included. As a result of our studies we propose a procedure with a lower number of steps than those previously described in the literature. This method has been applied to the analysis of nalidixic, 7-hydroxymethylnalidixic and oxolinic acids and cinoxacin in trout muscle. These analysis have been carried out using an HPLC system equipped with a C18 column and fluorimetric detection. The mobile phase was acetonitrile:oxalic acid. The recoveries obtained were: 70–97% for 7-hydroxymethylnalidixic acid, 75–78% for nalidixic acid, 71–95% for oxolinic acid and 72–85% for cinoxacin.

Key Words

Column liquid chromatography Quinolonic and cinolonic antibiotics Trout Fish farming 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • I. Durán Merás
    • 1
  • T. Galeano Díaz
    • 1
  • F. Salinas López
    • 1
  • M. I. Rodríguez Cacéres
    • 1
  1. 1.Department of Analytical ChemistryUniversity of ExtremaduraBadajozSpain

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