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An investigation into the application of capillary electrochromatography-mass spectrometry (CEC-MS) for the analysis and quantification of a potential drug candidate in extracted plasma

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Summary

Capillary Electrochromatography coupled to mass spectrometry offers a highly efficient and potentially sensitive separation and detection technique. Work presented demonstrates the separation of thirteen structurally related compounds from the parent drug candidate. The analysis of solid phase extracts of plasma samples containing known concentrations of drug are used to investigate the quantitative capabilities of the technique. Results and analysis protocols are discussed critically. Discussion of the instrumental layout and modifications and implications for future instrument design are also presented.

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References

  1. J.H. Knox, I.H. Grant, Chromatographia24, 135 (1987).

    CAS  Google Scholar 

  2. J.H. Knox, Chromatographia,26, 329 (1988).

    Article  CAS  Google Scholar 

  3. J.H. Knox, I.H. Grant, Chromatographia,32, 317 (1991).

    Article  CAS  Google Scholar 

  4. H. Yamamoto, J. Baumann, F. Erni, J. Chromatogr.,593, 317 (1992).

    Article  Google Scholar 

  5. N.W. Smith, M.B. Evans, Chromatographia,38, 317 (1994).

    Article  Google Scholar 

  6. R. Boughtflower, C. Paterson, T. Underwood, Chromatographia40, 329 (1995).

    Article  CAS  Google Scholar 

  7. R. Boughtflower, T. Underwood, J. Maddin, Chromatographia41, 398 (1995).

    Article  CAS  Google Scholar 

  8. C. Yan, R. Dadoo, H. Zhao, R.N. Zare, D.J. Rakestraw, Anal. Chem.,67, 2026 (1995).

    Article  CAS  Google Scholar 

  9. M. Dulay, C. Yan D. Rakestraw, R. Zare, J. Chromatogr.,725, 361 (1996).

    Article  CAS  Google Scholar 

  10. J.H. Knox, M. Saleem, J. Chromatogr. Sci.,7, 614 (1969).

    CAS  Google Scholar 

  11. J.H. Knox, M.T. Gilbert, J. Chromatogr. 186, 405 (1979).

    Article  CAS  Google Scholar 

  12. J.H. Knox, J. Chromatogr. Sci.,18, 453 (1980).

    CAS  Google Scholar 

  13. K. Karlsson, M. Novotny, Anal. Chem.60, 1662 (1988).

    Article  CAS  Google Scholar 

  14. S. Hoffmann, L. Blomberg, Chromatographia24, 416 (1987).

    CAS  Google Scholar 

  15. C. Yan, D. Schaufelberger, F. Erni, J. Chromatogr.,670, 15 (1994).

    Article  CAS  Google Scholar 

  16. K. Slais, D. Kourilova, J. Chromatogr.258, 57 (1983).

    Article  CAS  Google Scholar 

  17. S.J. Lane, R. Boughtflower, C. Paterson, T. Underwood, Rapid Comm. Mass Spec,9, 1283 (1995).

    Article  CAS  Google Scholar 

  18. S.J. Lane, R. Boughtflower, C. Paterson, M. Morris, Rapid Comm. Mass Spec.10, 733 (1996).

    Article  CAS  Google Scholar 

  19. H. Rebscher, U. Pyell, Chromatographia38, 737 (1994).

    Article  CAS  Google Scholar 

  20. F. Lelievre, C. Yan, R. Zare, P. Gareil, J. Chromatogr.723, 145 (1996).

    Article  Google Scholar 

  21. J. Pesek, M. Matyska, J. Chromatogr.736, 313 (1996).

    Article  CAS  Google Scholar 

  22. H. Rebscher, U. Pyell, J. Chromatogr.737, 171 (1996).

    Article  CAS  Google Scholar 

  23. J. Ding, P. Vouros, Anal. Chem.69, 379 (1997).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Separation Science Group, Glaxo Wellcome, Gunnels Wood Road, Stevenage, Herts, UK

    C. J. Paterson & R. J. Boughtflower

  2. Research Bio-Met, Glaxo Wellcome, Gunnels Wood Road, Stevenage, Herts, UK

    D. Higton & E. Palmer

Authors
  1. C. J. Paterson
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  2. R. J. Boughtflower
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  3. D. Higton
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  4. E. Palmer
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Paterson, C.J., Boughtflower, R.J., Higton, D. et al. An investigation into the application of capillary electrochromatography-mass spectrometry (CEC-MS) for the analysis and quantification of a potential drug candidate in extracted plasma. Chromatographia 46, 599–604 (1997). https://doi.org/10.1007/BF02490519

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  • DOI: https://doi.org/10.1007/BF02490519

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