Chromatographia

, Volume 51, Issue 7–8, pp 433–436 | Cite as

Development and validation of a liquid chromatography method for analysis of colistin sulphate

  • J. A. Orwa
  • A. Van Gerven
  • E. Roets
  • J. Hoogmartens
Originals

Summary

The peptide antibiotic Colistin has been successfully separated into more than thirty components on a YMC-Pack Pro, C-18 column with a mobile phase of acetonitrile—sodium sulphate (0.7% m/v)—phosphoric acid (6.8% v/v dilution of 85% m/m H3PO4)—water (21.5∶50∶5∶23.5) at 1.0 mL per min and detection at 215 nm. Baseline resolution of the major components, colistin A and B, and many minor components was obtained. Robustness was evaluated by performing a full-factorial design experiment. The method showed good selectivity, repeatability, linearity and sensitivity.

Key words

Column liquid chromatography Colistin Method validation 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • J. A. Orwa
    • 1
  • A. Van Gerven
    • 1
  • E. Roets
    • 1
  • J. Hoogmartens
    • 1
  1. 1.Faculteit Farmaceutische Wetenschappen, Laboratorium voor Farmaceutische Chemie en Analyse van GeneesmiddelenKatholieke Universiteit LeuvenLeuvenBelgium

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