, Volume 51, Issue 7–8, pp 417–427 | Cite as

Prediction of the chromatographic behaviour for a series of diuretic compounds

  • J. Barbosa
  • R. Bergés
  • V. Sanz-Nebot


The proportion of organic modifier and the pH of the acetonitrile-water mixtures used as mobile phases were optimized in order to separate a group of diuretic compounds covering a wide range of physyco-chemical properties. The Linear Solvation Energy Relationship (LSER) formalism based either on the multiparameter π*, β and α scales or the single solvent polarity parameterE T N , have been used to predict their chromatographic behaviour as a function of the percentage of acetonitrile in the eluent. Moreover, correlation established between retention and pH of the aqueous-organic mobile phases have been used to predict the chromatographic behaviour of the diuretic compounds studied as a function of the eluent pH. Linear correlation between a function of the eluent pH. Linear correlation between the chromatographic retention and theE T N polarity parameter of mobile phases containing different percentages of organic modifier has been obtained Based on the knowledge of the acid-base dissociation constant the relation between retention and mobile phase pH has also been linearized. These relationship allowed an important reduction of the experimental retention data needed for developing a given separation and a great improvement in chromatographic optimization schemes.

Key words

Column liquid chromatography Retentionvs mobile phase composition Retention prediction Diuretics 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • J. Barbosa
    • 1
  • R. Bergés
    • 1
  • V. Sanz-Nebot
    • 1
  1. 1.Departament de Química AnalíticaUniversitat de BarcelonaBarcelonaSpain

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