, Volume 52, Issue 3–4, pp 175–178 | Cite as

Separation of some flavonoids using RP-HPLC-NP-TLC off-line coupled system

  • M. A. Hawryl
  • E. Soczewinski
Originals Column Liquid Chromatography


Methods of separating a test mixture of 10 flavonoids were investigated using isocratic RP-HPLC, gradient RP-HPLC and a coupled isocratic RP-HPLC-NP-TLC system. Flavonoid fractions partially separated on an RP column were collected, evaporated, applied to a silica TLC plate and developed using 3-step gradient elution with methanol-ethyl acetate mobile phase.

Complete separation of the investigated compounds was by application of coupled RP-HPLC and NP-TLC.

Key Words

Column liquid chromatography Thin-layer chromatography Coupled chromatographic systems Two-dimensional chromatography Flavonoids 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • M. A. Hawryl
    • 1
  • E. Soczewinski
    • 1
  1. 1.Department of inorganic and Analytical ChemistryMedical AcademyLublinPoland

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