, Volume 53, Issue 5–6, pp 331–333 | Cite as

Determination of the endocrine disrupter bisphenol—A in the blood of uremia patients treated by dialysis

  • H. Shintani
Short Communications Column Liquid Chromatography


Automated solid-phase extraction (SPE) then high-performance liquid chromatography have been studied for determination of the endocrine disrupter bisphenol-A (BPA) in blood. Electrochemical detection was used for selective and sensitive detection of BPA. Determination of BPA in the blood of uremia patients treated by dialysis has not yet been reported. Acidified blood and acidified SPE eluent were used to suppress the ionization of BPA and thus retain the compound on a C18 column. Because artificial dialysis can be performed for periods longer than 20 years, for reasons of safety the amount of BPA migrating from the artificial dialyzer into the blood of a uremia patient was compared for different sterilization methods and for different artificial dialyzers manufactured from different materials.

Key Words

Column liquid chromatography Solid-phase extraction Dialysis Bisphenol-A 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • H. Shintani
    • 1
  1. 1.National Institute of Health SciencesTokyoJapan

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