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Chromatographia

, Volume 53, Issue 5–6, pp 326–330 | Cite as

4:3-β-naphthapyrone-4-acetic acidN-hydroxysuccinimidyl ester as a fluorescent labeling reagent for amino acids and oligopeptides in high-performance liquid chromatography

  • Xin Liu
  • Hong Wang
  • Shu-Cai Liang
  • Hua-Shan Zhang
Short Communications Column Liquid Chromatography

Summary

4:3-β-Naphthapyrone-4-acetic acidN-hydroxysuccinimidyl ester (NPA-OSu) is a highly sensitive and moderately reactive derivatizing reagent with a naphthapyrone moiety as fluorophore and anN-hydroxysuccinimidyl active ester as reactive group toward amino compounds. It is readily prepared in two steps. The fluorescence properties of NPA-OSu and its hydrolysis product have been studied in detail, and the conditions for derivatization and separation of the NPA-OSu derivatives of some amino acids and oligopeptides have been investigated. Atλ ex = 352 nm andλ em = 422 nm the detection limits (signal-to-noise ratio=3) for amino acids and oligopeptides reached fmol levels, for injection of 20 μL; this sensitivity was comparable with that obtained by use of 7-(diethylamino)coumarin-3-carboxylic acid succinimidyl ester as derivatizing reagent in the analysis of amino acids by capillary electrophoresis with laser-induced-fluorescence detection.

Key Words

Column liquid chromatography 4:3-β-Naphthapyrone-4-acetic acidN-hydroxysuccinimidyl ester (NPA-OSu) Amino acid Oligopeptide Fluorescent derivatizing eagent 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • Xin Liu
    • 1
  • Hong Wang
    • 1
  • Shu-Cai Liang
    • 1
  • Hua-Shan Zhang
    • 1
  1. 1.Department of ChemistryWuhan UniversityWuhanChina

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