Salting-out thin-layer chromatography of some angiotensin converting enzyme inhibitors on different sorbents
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Chromatographic behaviour of six antihypertensive drugs from the class ofACE inhibitors was studied under conditions of salting-out thin-layer chromatography on silica-gel, cellulose and polyacrylonitrile (PANS) as sorbents. For this purpose, different concentrations of aqueous ammonium sulphate solutions were used as solvents. In all cases studied, it was established that hR F values decrease with increasing salt concentrations in the chromatographic solvent systems used. In addition, a linear relationship of the correspondingR M values and ammonium sulphate content in the solvent was observed. To explain these results, a separation mechanism based on hydrophobic interaction between the sorbent and sorbent is proposed.
Key WordsThin-layer chromatography Salting-out ACE inhibitors Polyacrylonitrile sorbent Silica-gel Cellulose
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