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Chromatographia

, Volume 53, Supplement 1, pp S380–S386 | Cite as

Determination of organochlorine pesticides and chlorobenzenes in fruit and vegetables using subcritical water extraction combined with sorptive enrichment and CGC-MS

  • L. Wennrich
  • P. Popp
  • J. Breuste
Originals Gas Chromatography

Summary

A scheme has been developed for the determination of hexachlorocyclohexanes, DDX compounds and chlorobenzenes in fruit and vegetable samples (strawberries, apples, kohlrabi, lettuce and tomatoes). The method is based on the accelerated solvent extraction (ASE) with water or water-acetone mixtures followed by solid-phase microextraction (SPME) and stir bar sorptive extraction (SBSE), and subsequent thermodesorption-CGC-MS. Using spiked water and strawberry samples, important SPME and SBSE as well as ASE parameters were optimized. The ASE-SPME and ASE-SBSE procedures developed and optimized for the matrix strawberry could be applied successfully to other fruits and vegetables (apples, kohlrabi, lettuce and tomatoes). Both procedures were applied to the different matrices and evaluated. The relative standard deviations (n=5) for the analytes in different plant materials were in the range of 4 to 31% for both procedures. For the ASE-SPME procedure detection limits (LOD) between <0.5 and 6 μg kg−1 were achieved except for the DDX compounds (up to 40 μg kg−1). Using the ASE-SBSE, the LOD values for most of the analytes could be significantly improved. These procedures were applied to the determination of chlorobenzenes, HCH isomers and DDX compounds in strawberry, apple, kohlrabi, lettuce and tomato samples take from 12 allotment gardens in a potentially polluted region of East Germany.

Key Words

Gas chromatography-mass spectrometry Accelerated solvent extraction Chlorinated pesticides Subcritical water Fruit and vegetables 

References

  1. [1]
    Lino, C.M.; Noronha da Silveira, M.I.J. Chromatogr. A 1999,769, 275.CrossRefGoogle Scholar
  2. [2]
    Böhme, F.; Welsch-Pausch, K.; McLachlan, M.S.Environ. Sci. Technol. 1999,33, 1805.CrossRefGoogle Scholar
  3. [3]
    Lopez-Avila, V.; Young, R.; Benedicto, J.; Ho, P.; Kim, R.; Beckert, W.F.Anal. Chem. 1995,67, 2096.CrossRefGoogle Scholar
  4. [4]
    Lopez-Avila, V.; Young, R.; Kim, R.; Beckert, W.F.J. Chromatogr. Sci. 1995,33, 481Google Scholar
  5. [5]
    Wenzel, K.D.; Weißflog, L.; Paladini, E.; Gantuz, M.; Guerreiro, P.; Puliafito, C.; Schüürmann, G.Chemosphere 1997,34, 2505.CrossRefGoogle Scholar
  6. [6]
    Wenzel, K.D.; Hubert, A.; Manz, M.; Weissflog, L.; Engewald, W.; Schüürmann, G.Anal. Chem. 1998,70, 4827.CrossRefGoogle Scholar
  7. [7]
    Umlauf, G.; Hauk, H.; Reissinger, M.Chemosphere 1994, 28, 1689.CrossRefGoogle Scholar
  8. [8]
    Barnabas, I.A.; Dean, J.R.Analysist 1994,119, 2381.CrossRefGoogle Scholar
  9. [9]
    Camel, V.Analusis 1998,26 M99.CrossRefGoogle Scholar
  10. [10]
    Obana, H.; Kikuchi, K.; Okihashi, M.; Hori, S.Analyst 1997,122, 217.CrossRefGoogle Scholar
  11. [11]
    Okihashi, M.; Obana, H.; Hori, S.Analyst 1998,123, 711.CrossRefGoogle Scholar
  12. [12]
    Pawliszyn, J. “Solid-phase microextraction —theory and practice” Wiley-VCH, New York,1997.Google Scholar
  13. [13]
    Lord, H.; Pawliszyn, J.J. Chromatogr. A 2000,885, 153.CrossRefGoogle Scholar
  14. [14]
    Baltussen, E.; Sandra, P.; David, F.; Cramers, C.J. Microbolum Separations 1999,11, 737.CrossRefGoogle Scholar
  15. [15]
    Hawthorne, S.B.; Yang, Y.; Miller, D.J.Anal. Chem. 1994,66, 2912.CrossRefGoogle Scholar
  16. [16]
    Hageman, K.J.; Mazeas, L.; Grabansky, C.B.; Miller, D.J.; Hawthorne S.B.Anal. Chem. 1996,68, 3892.CrossRefGoogle Scholar
  17. [17]
    Hawthorne, S.B.; Grabanski, C.B.; Hageman, K.J.; Miller, D.J.J. Chromatogr. A 1998,814, 151.CrossRefGoogle Scholar
  18. [18]
    Wennrich, L.; Popp, P.; Köller, G.; Breuste, J.J. AOAC Int., submitted.Google Scholar
  19. [19]
    Popp, P.; Paschke, A.Chromatographia 1999,49, 686.CrossRefGoogle Scholar
  20. [20]
    Wennrich, L.; Popp, P.; Möder, M.Anal. Chem. 2000,72, 546.CrossRefGoogle Scholar
  21. [21]
    Borsdorf, H.; Opp, C.; Stach, J.Chemische Technik 1996,48, 92.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • L. Wennrich
    • 1
  • P. Popp
    • 2
  • J. Breuste
    • 1
  1. 1.Department of Urban LandscapesUFZ Centre for Environmental Research Leipzig/HalleLeipzigGermany
  2. 2.Department of Analytical ChemistryUFZ Centre for Environmental Research Leipzig/HalleLeipzigGermany

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