Summary
A rapid and accurate reversed-phase ion-pairing liquid chromatographic method with UV detection was developed for the simultaneous assay of the angiotensin-converting enzyme inhibitors benazepril hydrochloride, fosinopril sodium and ramipril, and the diuretic hydrochorothiazide.
The separation was achieved on a LC-8 (125×4.0 mml.D.; 5μm particle size) column.
The mobile phase consisted of 20 mM sodium heptanesulphonate (pH=2.5) and methanol (32:68v/v).
Validation of the method showed it to be precise, accurate and linear over the concentration range of analysis with a limit of detection of 1 ng for hydrochorothiazide, 2 ng for ramipril and benazepril and 8 ng for fosinopril.
The method developed was applied to the analysis of three different binary commercial formulations.
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Manna, L., Valvo, L. & Alimonti, S. A liquid chromatographic lon-pairing method for simultaneous determination of benazepril hydrochloride, fosinopril sodium, ramipril and hydrochlorothiazide in pharmaceutical formulations. Chromatographia 53 (Suppl 1), S271–S275 (2001). https://doi.org/10.1007/BF02490341
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DOI: https://doi.org/10.1007/BF02490341