Summary
On-line solid-phase extraction (SPE) coupled with reversed-phase liquid chromatography and UV detection at 254 nm has been used for the determination of trace-level polycyclic aromatic hydrocarbons (PAH) in soil extracts. Five commercially available adsorbents (C8, C18, PLRP-S, PRP-1, and Bond-Elut Env) were evaluated. Results showed that recovery of the PAH decreased with increasing molecular weight, because of their poorer solubility. Recovery of high-molecular-weight PAH was significantly improved by addition of 10% (v/v) acetonitrile to the sample before loading of the SPE adsorbent. PAH recovery ranged from 64.0 to 108% when a 50 mL sample spiked with 1 μg L−1 was applied to these adsorbents. Determination of PAH was possible with detection limits below 0.05 μg L−1, which corresponds to 0.2 μg kg−1 soil. The method was successfully used to determine PAH in soil extracts.
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References
Misfeld, J.; Timm, J.,J. Environ. Pathol. Toxicol. 1998,1, 747.
Kiss, G.; Varga-Puchony, Z.; Hlavay, J.J. Chromatogr. A 1996,725, 261.
Bonfanti, L.; Careri, M.; Mangia, A.; Maspero, M.J. Chromatogr. A 1996,728, 359.
Morabito, P.L.; McCabe, T.; Hiller, F.J.; Zakett, D.J. High Resol. Chromatogr. 1993,16, 90.
Gremm, T.J.; Frimmel, F.H.Chromatographia 1994,38, 781.
Berset, J.D.; Ejem, M.; Holzer, R.; Lischer, P.Anal. Chim. Acta 1999,383, 263.
Barnabas, I.J.; Dean, J.R.; Tomlinson, W.R.; Owen, S.P.,Anal. Chem. 1995,67, 2064.
Kootstra, P.R.; Straub, M.H.C.; Stil, G.H.; Vander Velde, E.G.; Hesselink, W.; Land, C.C.,J. Chromatogr. A 1995,697, 123.
Sargenti, S.R.; McNair, H.M.J. Microcol. Sep. 1998,10, 125.
Rein, J.; Cork, C.M.; Furton, K.G.J. Chromatogr. 1991,545, 149.
Barceló, D.; Henion, M.-C.Trace Determination of Pesticides and their Degradation Products in Water, Elsevier, Amsterdam,1997, pp. 243–256.
Henion, M.-C.J. Chromatogr. A 1999,856, 3.
Marcé, R.M.; Borrull, F.J. Chromatogr. A 2000,885, 273.
Brouwer, E.R.; Hermans, A.N.J.; Lingeman, H.; Brinkman, U.A.Th.J. Chromatogr. A 1994,669, 45.
Zouzige, M.; Pichon, V.; Hennion, M.-C.J. Chromatogr. A 1998,823, 197.
Harrak, R.El.; Calull, M.; Marcé, R.M.; Borrull, F.J. High Resol. Chromatogr. 1998,21, 667.
Masqué, N.; Marcé, R.M.; Borrull, F.Trends Anal. Chem. 1998,17, 384.
Thurman, E.M., Mills, M.S.Solid-phase Extraction. Principles and Practice, Wiley, New York,1998.
Pichon, V.J. Chromatogr. A 2000,885, 195.
Wissiack, R.; Rosenberg, E.; Grasserbauer, M.J. Chromatogr. A 2000,896, 159.
Huck, C.W.; Bonn, G.K.J. Chromatogr. A 2000,885, 51.
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Chen, Z.L., Megharaj, M. & Naidu, R. Comparison of adsorbents for on-line solid-phase extraction of polycyclic aromatic hydrocarbons before liquid chromatography with UV detection. Chromatographia 56, 105–108 (2002). https://doi.org/10.1007/BF02490254
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DOI: https://doi.org/10.1007/BF02490254